07-428-I

Anti-phospho eNOS Antibody (Ser1177)

from rabbit, purified by affinity chromatography

• 別名: Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), porcine (based on 100% sequence homology), sheep (based on 100% sequence homology), canine (based on 100% sequence homology)
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_000594
• UniProtアクセッション番号: P29474
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer1177)
• 遺伝子情報: human ... NOS3(4846)

詳細

詳細

NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

免疫原

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human eNOS phosphorylated at Ser1177.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経変性疾患

Anti-phospho eNOS Antibody (Ser1177) is a highly specific rabbit polyclonal antibody, that targets eNOS/NOS III & has been tested in western blotting.

品質

Evaluated by Western Blotting in untreated and VEGF treated HUVEC cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected phospho eNOS (Ser1177) in 10 µg of VEGF treated HUVEC cell lysate.

ターゲットの説明

~133 kDa observed

関連事項

Replaces: 07-428

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable