Here is the link to our Biochemical Solubility table:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/287/917/c3045inf.pdf
For the Cell Culture Tested grade of this product, C3045, three options are provided: Serum, Tween and Ethanol.
Note that if Serum is used as the solvent, it may already contain some Cholesterol. The "Tween" is referring to an aqueous solution of either TWEEN 20 or TWEEN 80. Also, Ethanol can be used to make a stock solution, before supplementing the culture medium; consider perhaps a 100X solution in ethanol, which would give a concentration of 1 percent ethanol in the medium.
C8667
Colesterolo
Sigma Grade, ≥99%
Cholesterol
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Sinonimo/i:
3β-idrossi-5-colestene, 5-colestene-3β-olo
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
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Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 500 mg | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 58,30 € | |
| 1 g | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 73,80 € | |
| 5 g | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 127,00 € | |
| 25 g | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 420,00 € | |
| 100 g | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 1.340,00 € |
Informazioni su questo articolo
Formula empirica (notazione di Hill):
C27H46O
Numero CAS:
Peso molecolare:
386.65
NACRES:
NA.77
PubChem Substance ID:
eCl@ss:
39023139
UNSPSC Code:
12352111
EC Number:
200-353-2
MDL number:
Beilstein/REAXYS Number:
1915888
58,30 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
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Permettici di aiutartibiological source
sheep wool
Quality Segment
grade
Sigma Grade
assay
≥99%
form
powder
bp
360 °C (lit.)
mp
147-149 °C (lit.)
solubility
water: soluble 0.00003 g/L at 20 °C
density
1.067 g/mL at 25 °C (lit.)
application(s)
advanced drug delivery
shipped in
ambient
storage temp.
−20°C
SMILES string
CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C
InChI
1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1
InChI key
HVYWMOMLDIMFJA-DPAQBDIFSA-N
Gene Information
human ... POLA1(5422), TOP2A(7153)
rat ... Ar(24208), Polb(29240)
Application
Standard per la cromatografia
Il colesterolo è stato usato per preparare complessi immunostimolanti per potenziare le risposte cellulari e umorali.[1] È stato incorporato nel mangime animale per studiare l′effetto della dieta ricca di colesterolo.
Biochem/physiol Actions
Componente principale di tutte le membrane biologiche; il colesterolo rappresenta il 25% circa dei lipidi cerebrali totali.
Il colesterolo è un lipide che rappresenta fino a 20-25% circa dei componenti strutturali delle membrane cellulari. Determina la fluidità e la permeabilità della membrana, rendendola permeabile all′acqua ma non a ioni e protoni. Il colesterolo regola anche le funzioni dei trasportatori e delle proteine di segnale presenti sulla membrana citoplasmatica.[2] Le principali sedi di sintesi del colesterolo sono l′intestino tenue e il fegato.[3]
Preparation Note
In alcol riscaldato il colesterolo monoidrato produce una soluzione di limpida, da incolore a giallo chiaro. È anche solubile in acetone, diossano, etilacetato, benzene, etere di petrolio, oli, grassi e soluzioni acquose di sali biliari. Le soluzioni devono essere conservate al riparo dalla luce.
1 of 1
Questo articolo | |||
|---|---|---|---|
| grade Sigma Grade | grade - | grade - | grade - |
| assay ≥99% | assay - | assay ≥99% | assay ≥92.5% (GC) |
| biological source sheep wool | biological source - | biological source ovine wool | biological source sheep wool |
| form powder | form powder | form powder | form powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. room temp |
| shipped in ambient | shipped in - | shipped in ambient | shipped in ambient |
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Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articoli
Cholesterol biosynthesis starts in the hepatic endoplasmic reticulum with acetyl-CoA, yielding 3-hydroxy-3-methylglutaryl-CoA via HMG-CoA synthase.
Contenuto correlato
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| C8667-5G | 04061835543632 |
| C8667-100G | 04061835543601 |
| C8667-500MG | 04061835543625 |
| C8667-25G | 04061835521746 |
| C8667-1G | 04061835543618 |
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how to prepare for cell culture? thank you
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What is the best temperature for storage for 5 mg/mL solutions in ethanol? Can they be stored at 4C? Thank you!
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The stability of this material in solution at 2-8°C has not been evaluated. Solutions prepared in Chloroform, in aliquots and protected from light, are stable for at least 1 year when stored at -20°C. Solutions prepared in Ethanol are expected to be similarly stable.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfUtile?
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Buongiorno, volevo chiedere cortesemente quale sia l'origine del prodotto
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This product is derived from sheep wool. The origin information of this product will be listed on the lot-specific Certificate of Origin. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under the ‘Certificate of Origin’ tab:
https://www.sigmaaldrich.com/product/sigma/c8667#product-documentationUtile?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Hi, I want to know if this is from plant or sheep wool?
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As mentioned in the PROPERTIES section of the product page, this material is from sheep wool.
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