IP02
Protein A Agarose Suspension
Protein A Agarose Suspension designed for immunoprecipitation applications
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Informazioni su questo articolo
NACRES:
NA.56
UNSPSC Code:
41116133
Prezzi e disponibilità al momento non sono disponibili
Servizio Tecnico
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Permettici di aiutartiform
slurry (Liquid)
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
shipped in
wet ice
storage temp.
2-8°C
1 of 1
Questo articolo | |||
|---|---|---|---|
| form slurry (Liquid) | form slurry (Liquid) | form slurry (Liquid) | form aqueous suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| contains ≤0.1% sodium azide as preservative | contains ≤0.1% sodium azide as preservative | contains ≤0.1% sodium azide as preservative | contains - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename - |
| storage condition do not freeze | storage condition do not freeze | storage condition do not freeze | storage condition - |
General description
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG2a and IgG2b and rabbit IgG.
Protein A conjugated to agarose.
Physical form
In PBS.
Other Notes
Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG2a or IgG2b monoclonal antibodies. Mouse IgG1 or IgG3 and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenuto correlato
We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| IP02-1.5ML | 04055977220209 |
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