S7897
Sarcosine Oxidase from Bacillus sp.
lyophilized powder, 25-50 units/mg solid
Sinonimo/i:
Sarcosine: oxygen oxidoreductase (demethylating)
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Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 1000 units | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 589,00 € |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-850-5
MDL number:
Specific activity:
25-50 units/mg solid
589,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
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Permettici di aiutartiform
lyophilized powder
Quality Level
specific activity
25-50 units/mg solid
mol wt
44 kDa
storage temp.
−20°C
General description
Monomeric sarcosine oxidase (MSOX) is a flavoenzyme that catalyzes the oxidative demethylation of sarcosine (N-methylglycine) to yield glycine, formaldehyde, and hydrogen peroxide. Monomeric sarcosine oxidase can oxidize other secondary amino acids such as N-methyl-L-alanine, N-ethylglycine, and L-proline. [1]
Sarcosine oxidase is a 44 kDa protein with covalently bound 1 mol of flavin adenine dinucleotide (FAD). It belongs to the family of amino acid oxidases with bound FAD.
Application
Sarcosine oxidase has been used in a study as part of a multienzyme cascade, that when immobilized constructed amperometric biosensors. [2] Sarcosine oxidase has also been used in a study to investigate oxidation of amines by flavoproteins. [3]
Sarcosine oxidase from Bacillus sp. has been used as a positive control for detection of proteins with covalently bound flavin adenine dinucleotide (FAD) by UV. It has also been used in co-immobilization with other enzymes on platinum electrode for preparation of creatinine biosensor.[4]
Physical form
No stabilizers added
Other Notes
One unit will form 1.0 μmole of formaldehyde from sarcosine per min at pH 8.3 at 37 °C.
1 of 1
Questo articolo | |||
|---|---|---|---|
| specific activity 25-50 units/mg solid | specific activity ≥0.45 units/mg protein | specific activity 8-20 units/mg solid | specific activity ≥3 units/mg solid, ≥40 U/mg |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 44 kDa | mol wt ~88 kDa by electrophoresis | mol wt 71 kDa by SDS-PAGE, 83 kDa by gel filtration | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Contenuto correlato
Rastislav Monošík et al.
Analytical biochemistry, 421(1), 256-261 (2011-11-10)
Amperometric biosensors based on gold planar or nanocomposite electrode containing multiwalled carbon nanotubes for determination of glycerol were developed. The biosensors were constructed by immobilization of a novel multienzyme cascade consisting of glycerol kinase/creatine kinase/creatinase/sarcosine oxidase/peroxidase between a chitosan "sandwich."
Paul F Fitzpatrick
Archives of biochemistry and biophysics, 493(1), 13-25 (2009-08-05)
Many flavoproteins catalyze the oxidation of primary and secondary amines, with the transfer of a hydride equivalent from a carbon-nitrogen bond to the flavin cofactor. Most of these amine oxidases can be classified into two structural families, the D-amino acid
Optimization of the production of Chondrus crispus hexose oxidase in Pichia pastoris
Wolff AM, et al.
Protein Expression and Purification, 22(2), 189-199 (2001)
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| S7897-1KU | 04061832731377 |