C4405
Choline Oxidase from Arthrobacter globiformis
lyophilized powder, 8-20 units/mg solid
Sinonimo/i:
Choline: oxygen 1-oxidoreductase
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About This Item
Prodotti consigliati
Forma fisica
lyophilized powder
Attività specifica
8-20 units/mg solid
PM
71 kDa by SDS-PAGE
83 kDa by gel filtration
Temperatura di conservazione
−20°C
Descrizione generale
Research area: Cell signaling. Choline oxidaseis a flavoprotein and belongs to the glucose methanol choline(GMC)-oxidoreductase family.
Applicazioni
Choline Oxidase from Arthrobacter globiformis has been used:
- for enzymatic determination of choline (Cho) and phosphocholine (P-Cho)
- in sphingomyelin assay to determine the concentration of sphingomyelin by measuring free choline
- in enzyme coating for the fully-assembled recording device for electrochemical measurements
Choline oxidase from Sigma has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection.Choline oxidase from Arthrobacterglobiformis is suitable for the preparation of amperometric biosensors.
Azioni biochim/fisiol
Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the investigation of the reaction mechanism for the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approximately 83,000 Da according to gel filtration or approximately 71,000 Da according to SDS gel electrophoresis. The optimum pH is found to be around pH 7.5 and the isoelectric point (pI) around pH 4.5.
Definizione di unità
One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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I clienti hanno visto anche
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Nuccio ML, et al.
Metabolic engineering, 2(4), 300-311 (2000)
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Disney AA, et al.
Journal of Neuroscience Methods, 255, 29-37 (2015)
Fan Fan et al.
Journal of the American Chemical Society, 127(7), 2067-2074 (2005-02-17)
Choline oxidase catalyzes the four-electron oxidation of choline to glycine betaine, with betaine aldehyde as an intermediate. In this study, primary deuterium and solvent kinetic isotope effects have been used to elucidate the mechanism for substrate oxidation by choline oxidase
Anish Khan et al.
Biosensors & bioelectronics, 31(1), 433-438 (2011-12-14)
The electrochemical biosensors based on poly(o-phenylenediamine) (PoPD) and acetylcholinesterase (AChE) and choline oxidase (ChO) enzymes were fabricated on carbon fibre (CF) substrate. The electropolymerized PoPD was used to reduce the interfering substances. The electrode assembly was completed by depositing functionalized
J L Lima et al.
The Analyst, 125(7), 1281-1284 (2000-09-14)
The development of a FIA system for the determination of total choline content in several types of milk is described. The samples were submitted to hydrochloric acid digestion before injection into the system and passed through an enzymatic reactor containing
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