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M0650

Terreno di coltura 199

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

Sinonimo/i:

Cell growth medium, M199 Medium, TCM 199

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Cambia visualizzazione
Taglio della confezioneSKUDisponibilitàPrezzo
100 mL
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
35,80 €

Informazioni su questo articolo

UNSPSC Code:
12352207
NACRES:
NA.75

35,80 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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Nome del prodotto

Terreno di coltura 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

General description

Il terreno di coltura M199 consiste in una combinazione di vitamine, amminoacidi e altri fattori ed è stato inizialmente sviluppato come terreno a formulazione completamente definita per la coltura di espianti primari. Se adeguatamente integrato, questo terreno trova ampia applicabilità, in particolare per coltivare cellule non trasformate. Il terreno M19 trova uso nella produzione di vaccini, nella coltura in vitro di espianti pancreatici primari e nei tessuti del cristallino.

Application

Medium 199 has been used:

  • to culture human mesothelial cells[1]
  • as a component of the holding medium for the collection of embryos from pluriparous Boer goat
  • in the preparation of spheroid-embedded collagen matrices[2]

Preparation Note

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

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Medium 199 With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

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Medium 199

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

powder

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

components

phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes

components

L-glutamine: yes
phenol red: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

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Classe di stoccaggio

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
RNBL7705
RNBL1645
RNBK2084
RNBJ1355
RNBH5449

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Articoli

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

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Numero articolo commerciale globale

SKUGTIN
M0650-100ML04061833993811

Domande

  1. If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?

    1 risposta

    1. Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
      Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
      (NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
      • Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
      • While gently stirring the water, add 100 ml of 10X medium.
      • To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
      • While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
      • Bring medium to final volume with additional tissue culture grade water.
      • It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
      • Aseptically dispense into sterile containers. Store refrigerated at 2-8° C

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