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Merck

D4010

2′-Deoxyguanosine 5′-triphosphate sodium salt hydrate

≥96% (HPLC), synthetic (organic), powder

Sinonimo/i:

2′-deoxy-, 5′-triphosphate sodium salt, Guanosine, dGTP sodium salt hydrate

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Informazioni su questo articolo

Formula empirica (notazione di Hill):
C10H16N5O13P3 · xNa+ · yH2O
Numero CAS:
Peso molecolare:
507.18 (anhydrous free acid basis)
MDL number:
UNSPSC Code:
41106305
NACRES:
NA.51
PubChem Substance ID:
Assay:
≥96% (HPLC)
Biological source:
synthetic (organic)
Form:
powder
Storage temp.:
−20°C

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Nome del prodotto

2′-Deoxyguanosine 5′-triphosphate sodium salt hydrate, ≥96% (HPLC)

biological source

synthetic (organic)

Quality Level

assay

≥96% (HPLC)

form

powder

shipped in

dry ice

storage temp.

−20°C

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Questo articolo
D7409D5038D7170
biological source

synthetic (organic)

biological source

-

biological source

-

biological source

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

assay

≥96% (HPLC)

assay

≥90% (HPLC)

assay

≥99%

assay

≥99%

form

powder

form

powder

form

liquid

form

liquid

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

2′-Deoxyguanosine 5′-triphosphate sodium salt hydrate has been used:
  • as a component in a polymerase chain reaction (PCR) mixture for PCR product formation using AM-toxin primers[1]
  • as an internal standard to improve the analytical performance of capillary electrophoresis (CE) analysis for the evaluation of the cleanup process[2]
  • as a putative substrate in in vitro enzyme activity assay to incubate with nudix hydrolase 15 (NUDT15) protein for the evaluation of putative NUDT15 inhibitors[3]

Biochem/physiol Actions

Deoxyguanosine 5′-triphosphate (dGTP), a purine deoxynucleotide, is a substrate of DNA polymerase(s) and reverse transcriptases. dGTP is useful to study the distribution, specificity, and kinetics of various nucleoside-triphosphatases (NTPase) and ribonucleotide reductases.

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)


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Chikungunya virus (CHIKV) is an insect borne virus (genus: Alphavirus) which causes acute febrile illness in humans followed by a prolonged arthralgic disease that affects the joints of the extremities. Re-emergence of the virus in the form of outbreaks in
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During DNA synthesis, DNA polymerases must select against ribonucleotides, present at much higher levels compared with deoxyribonucleotides. Most DNA polymerases are equipped to exclude ribonucleotides from their active site through a bulky side chain residue that can sterically block the
Inchul Yang et al.
Analytical biochemistry, 335(1), 150-161 (2004-11-03)
A novel approach for quantitation of DNA (oligonucleotides) with an unprecedented accuracy of approximately 1% is reported. Quantitation of DNA is commonly performed by measuring UV absorption or fluorescence from dyes intercalated into DNA. Both methods need accurate quantitation standards
Yimon Aye et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(24), 9815-9820 (2011-06-02)
Human ribonucleotide reductases (hRNRs) catalyze the conversion of nucleotides to deoxynucleotides and are composed of α- and β-subunits that form active α(n)β(m) (n, m = 2 or 6) complexes. α binds NDP substrates (CDP, UDP, ADP, and GDP, C site)
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Ganciclovir (GCV) is the first-line therapy against human cytomegalovirus (HCMV), a widespread infection that is particularly dangerous for immunodeficient individuals. Closely resembling deoxyguanosine triphosphate, the tri-phosphorylated metabolite of GCV (GCV-TP) is preferentially incorporated by the viral DNA polymerase, thereby terminating

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