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D0547

Sigma-Aldrich

Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Sinonimo/i:

DME/F-12, miscela 1:1

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About This Item

Numero MDL:
MFCD00217417
Codice UNSPSC:
12352207
NACRES:
NA.71

Livello qualitativo

500

Forma fisica

powder

tecniche

cell culture | mammalian: suitable

Componenti

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Descrizione generale

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Applicazioni

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

  • to culture cerebral cortices from mice embryos
  • as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
  • to culture human corneal epithelial cell lines

Altre note

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

Quantità

Formulated to contain 12.0 grams of powder per liter of medium.

Ricostituzione

Supplement with 1.2 g/L sodium bicarbonate

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Diplodiosis is an important neuromycotoxicosis of ruminants in South Africa when grazing on harvested maize fields in winter. It is believed to be caused by mycotoxin(s) synthesised by Stenocarpella (Diplodia) maydis. Although several metabolites have been isolated from S. maydis
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PPARGC1A encodes a transcriptional co-activator also termed peroxisome proliferator-activated receptor (PPAR) gamma coactivator 1-alpha (PGC-1α) which orchestrates multiple transcriptional programs. We have recently identified CNS-specific transcripts that are initiated far upstream of the reference gene (RG) promoter. The regulation of
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Articoli

Nutrient Mixture F-12 Ham Formulation

Nutrient Mixture F-12 Ham Formulation

Protocolli

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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