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B8781

Restriction Enzyme Buffer SB

10 ×

Sinonimo/i:

Restriction Endonuclease Buffer

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5 x 1 vial
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58,20 €

Informazioni su questo articolo

NACRES:
NA.53
UNSPSC Code:
12352204

58,20 €

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grade

Molecular Biology

Quality Segment

200

description

10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl2, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.

form

buffered aqueous solution

concentration

10 ×

storage temp.

−20°C

SMILES string

CC(C)CC(CNC(Cc1ccccc1)C(N)=O)NC(=O)C(Cc2cnc[nH]2)NC(=O)CNC(=O)C(NC(=O)C(C)NC(=O)C(Cc3c[nH]c4ccccc34)NC(=O)C(CCC(N)=O)NC(=O)C(N)Cc5c[nH]c6ccccc56)C(C)C

InChI

1S/C58H77N15O9/c1-32(2)21-39(29-65-46(52(61)76)22-35-13-7-6-8-14-35)69-57(81)48(25-38-28-62-31-67-38)70-50(75)30-66-58(82)51(33(3)4)73-53(77)34(5)68-56(80)47(24-37-27-64-44-18-12-10-16-41(37)44)72-55(79)45(19-20-49(60)74)71-54(78)42(59)23-36-26-63-43-17-11-9-15-40(36)43/h6-18,26-28,31-34,39,42,45-48,51,63-65H,19-25,29-30,59H2,1-5H3,(H2,60,74)(H2,61,76)(H,62,67)(H,66,82)(H,68,80)(H,69,81)(H,70,75)(H,71,78)(H,72,79)(H,73,77)

InChI key

YNZJHLFHFNCZIE-UHFFFAOYSA-N

General description

This buffer is for use with Sigma Restriction Enzymes. Please note every restriction enzyme is supplied with the appropriate buffer - this product is available for users who need additional buffer. For details on buffer composition and protocols for use, see the "Protocols and Articles" tab above.

Application

Restriction Enzyme Buffer SB has been used in restriction digestion of PCR amplified product.

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Sigma-Aldrich

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Sigma-Aldrich

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grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

concentration

10 ×

concentration

2 ×

concentration

-

concentration

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

form

buffered aqueous solution

form

buffered aqueous solution

form

solution

form

solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

description

10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl2, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.

description

2× buffer contains 200 mM Tris-HCl, pH 7.6, 20 mM MgCl2, 300 mM NaCl, 2 mM dithiothreitol at 37 °C.

description

Saline-Sodium Citrate buffer, made with ultrapure water

description

-

Classe di stoccaggio

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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