Passa al contenuto
Merck
Tutte le immagini(1)

Documenti

S2015

Sigma-Aldrich

SSPE Buffer 20× Concentrate

for Northern and Southern blotting, solution

Sinonimo/i:

20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Codice UNSPSC:
41105319
NACRES:
NA.25

Grado

for molecular biology

Livello qualitativo

200

Sterilità

0.2 μm filtered

Forma fisica

solution

Attività estranea

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

Temperatura di conservazione

room temp

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Applicazioni

Suitable for:
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for microarrays

Caratteristiche e vantaggi

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Componenti

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

D9156

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

D0428

1 kb DNA Ladder, for DNA electrophoresis

H7033

Tampone per ibridazione PerfectHyb™ Plus, for Northern and Southern blotting, solution

T9285

Tampone Tris-EDTA, 100X concentrate for Northern and Southern blotting

S6639

SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution

B6185

Bionic™ Buffer, 10X Concentrate, for Northern and Southern blotting, solution

GERPN3005

ECL™ Direct Nucleic Acid, Cytiva RPN3005, pack of 1 ea

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures.
Pierce SE, et al.
Nature Protocols, 2, 2958-2974 (2007)
Glucocorticoid antagonist RU38486 fails to block acid-induced muscle wasting in vivo or in vitro.
Pickering WP, et al.
Nephrology, Dialysis, and Transplantation, 18, 1475-1484 (2003)
Warren P Pickering et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 18(8), 1475-1484 (2003-08-05)
Increased protein degradation during metabolic acidosis contributes to muscle wasting in uraemia. Adrenalectomy experiments in severely acidotic rats (arterial pH approximately 7.15) have shown that this is prevented in the absence of glucocorticoid. It should therefore be possible to block
Claudia S Meier-Trummer et al.
PloS one, 4(7), e6265-e6265 (2009-07-16)
Malignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes in various tissues. Ovine gamma herpesvirus 2 (OvHV-2) is a causative agent of MCF, which may trigger the disease

Protocolli

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.