A3151
Avidin–Peroxidase
lyophilized powder
Sinonimo/i:
Avidin-POD
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2 MG
204,00 €
1 MG
335,00 €
5 MG
270,20 €
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Cambia visualizzazione
2 MG
204,00 €
1 MG
335,00 €
5 MG
270,20 €
About This Item
Prodotti consigliati
Origine biologica
avidin from egg white
enzyme from horseradish
Livello qualitativo
Coniugato
peroxidase conjugate
Stato
lyophilized powder
Composizione
Protein, ≥70% E1%/280
Grado di funzionalizzazione
0.7-2.0 mol peroxidase per mol avidin
tecniche
direct ELISA: 1:50,000
Solubilità
H2O: soluble 1 mg/mL
PBS: soluble
Temperatura di conservazione
−20°C
Descrizione generale
Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Applicazioni
Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding[2] and OVA-IgG1[3].
To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.
Stato fisico
Lyophilized powder containing citrate buffer.
Nota sulla preparazione
Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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