A2831

Anti-dinitrofenil

clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

• Sinonimo/i: Anticorpo monoclonale Anti-DNP
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: alkaline phosphatase conjugate
• Clone: SPE-7, monoclonal
• Application: DB, ELISA (d)
• Citations: 5

Proprietà

• biological source: mouse
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: SPE-7, monoclonal
• form: buffered aqueous glycerol solution
• technique(s): direct ELISA: 1:25,000 using DNP-BSA, dot blot: 1:180,000 (chemiluminescence)
• isotype: IgE
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Descrizione

General description

Dinitrophenyl (DNP) is a hapten that is used for labeling primary or secondary probes in immunological analyses. Thus, anti-DNP antibody conjugated to a detectable substrate is a useful tool for the study of target proteins. Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody is specific for DNP.

Immunogen

Coniugato DNP-KLH

Application

L′anticorpo può essere usato per l′induzione sperimentale di reazioni allergiche controllate o come fonte di IgE murine.

L′anticorpo può essere usato per l′induzione sperimentale di reazioni allergiche controllate o come fonte di IgE murine.Per indurre l′anafilassi sistemica, ai topi sono stati iniettati 3 μg di IgE di topo anti-DNP e quindi venti ore più tardi sono stati somministrati EV 500 μg di DNP-BSA. Per indurre l′anafilassi locale, ai topi sono stati iniettati per via intradermica 10 ng di IgE di topo anti-DNP e quindi è stata effettuata la provocazione con un′iniezione EV di 500 μg di DNP-BSA.

Usato per indurre reazioni allergiche controllate o come fonte di IgE murine.

Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody can be used for ELISA (1:25,000) and dot blot (1:180,000) assays.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% BSA, and 50% glycerol and 15 mM Sodium azide

Analysis Note

Monoclonal Anti-DNP is tested to ensure minimal reactivity against bovine serum albumin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Classe di stoccaggio: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)