MABC189

Anti-PIDD Antibody, clone 1511.1

clone 15F11.1, from mouse

• Sinonimo/i: p53-induced protein with a death domain, Leucine-rich repeat and death domain-containing protein, PIDD
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 15F11.1, monoclonal
• Reattività contro le specie: rat, mouse, human
• tecniche: immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_665893
• N° accesso UniProt: Q9HB75
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... PIDD1(55367)

Descrizione

Descrizione generale

p53-induced protein with a death domain (PIDD) is also called Leucine-rich repeat and death domain-containing protein (LRDD). PIDD is involved in apoptosis downstream of the tumor suppressor. PIDD is a component of the DNA damage/stress response pathway in which p53/TP53 is associated with apoptosis. PIDD also aids in the activation of the transcription factor NF-kappa-B, and CASP2 which regulates apoptotic pathways.

Specificità

This antibody demonstrates specificity for isoform 4, but may detect isoform 1, 2 and 3. It will not detect isoforms 5, 6, and 7 based upon immunogen design.

Immunogeno

Recombinant protein corresponding to human PIDD.

Applicazioni

Anti-PIDD Antibody, clone 1511.1 is a highly specific mouse monoclonal antibody, that targets p53-induced protein with a death domain & has been tested in western blotting & ICC.

Western Blotting Analysis: 1 µg/mL from a representative lot detected PIDD in 10 µg of NIH/3T3 and PC-12 cell lysate.

Immunohistochemistry Analysis: A 1:100 dilution, a 1:500 dilution, and a 1:1,000 dilution detected PIDD in HeLa, A431, and NIH/3T3 cells.

Qualità

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PIDD in 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~66 kDa observed. Uniprot describes 7 different isoforms.

Stato fisico

Format: Purified

Purified mouse IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable