APT110
Apo-Direct TUNEL Assay Kit
The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.
Sinonimo/i:
Apoptosis detection kit, TUNEL apoptosis assay, TUNEL assay kit
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| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|
Informazioni su questo articolo
UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
Prezzi e disponibilità al momento non sono disponibili
Servizio Tecnico
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Permettici di aiutartiQuality Level
species reactivity
vertebrates
manufacturer/tradename
ApoDIRECT, Chemicon®
technique(s)
flow cytometry: suitable
detection method
fluorometric
shipped in
wet ice
1 of 1
Questo articolo | |||
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| species reactivity vertebrates | species reactivity - | species reactivity - | species reactivity - |
| manufacturer/tradename ApoDIRECT | manufacturer/tradename - | manufacturer/tradename ApopTag, Chemicon® | manufacturer/tradename ApopTag, Chemicon® |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| Quality Level 100 | Quality Level 300 | Quality Level 100 | Quality Level 100 |
General description
The CHEMICON APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.
Preparation Note
Precautions and Warnings:
1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.
2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets.
3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.
1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.
2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets.
3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.
Other Notes
The following components are included with the APO-DIRECT Kit. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 50 cell suspensions including control cells of approximately 1 x 106 cells per suspension for flow cytometry analysis. The control cells have been fixed as described (under the heading cell fixation procedure) and are in 70% (v/v) ethanol.
Positive Control Cells,Brown cap
Negative Control Cells, Natural cap
Wash Buffer, Blue cap
Reaction Buffer, Green cap
TdT Enzyme, Yellow cap
F-dUTP, Orange cap
Rinsing Buffer, Red cap
PI/RNase Staining Buffer, Amber bottle
Positive Control Cells,Brown cap
Negative Control Cells, Natural cap
Wash Buffer, Blue cap
Reaction Buffer, Green cap
TdT Enzyme, Yellow cap
F-dUTP, Orange cap
Rinsing Buffer, Red cap
PI/RNase Staining Buffer, Amber bottle
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1
Classe di stoccaggio
3 - Flammable liquids
flash_point_f
69.8 °F
flash_point_c
21 °C
wgk
WGK 3
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| APT110 | 04053252001000 |
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