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05-613

Anti-β-Catenin Antibody, clone 2H4A7

clone 2H4A7, Upstate®, from mouse

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Taglio della confezioneSKUDisponibilitàPrezzo
200 μg

Disponibile per la spedizione OGGIdaMerck S.p.A

428,00 €

Informazioni su questo articolo

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2H4A7, monoclonal
Application:
IHC, IP, WB
Citations:
9

428,00 €


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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

2H4A7, monoclonal

species reactivity

rat, mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Ctnnb1(12387)

General description

92kDa

Immunogen

fusion protein corresponding to residues 1-100 of human β-Catenin

Application

Detect β-Catenin also known as beta catenin with Anti-β-Catenin Antibody, clone 2H4A7 (Mouse Monoclonal Antibody), that has been demonstrated to work in IP, WB & IHC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Biochem/physiol Actions

β-Catenin

Physical form

Format: Purified
Protein G Chromatography
Protein G purified IgG in buffer containing 0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide

Preparation Note

Stable 2 years at 2-8°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from A431, mouse 3T3/A31 or rat L6 cell lysates

Other Notes

Replaces: 04-1002

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Questo articolo
04-958MABF316105-665-AF647
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

ALEXA FLUOR 647

species reactivity

rat, mouse, human

species reactivity

rat, mouse, human

species reactivity

human

species reactivity

human, mouse

clone

2H4A7, monoclonal

clone

7F7.2, monoclonal

clone

12F7, monoclonal

clone

8E7, monoclonal

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

UniProt accession no.

P35222

UniProt accession no.

P35222

UniProt accession no.

O42486

UniProt accession no.

P35222


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Classe di stoccaggio

10 - Combustible liquids

wgk

WGK 1



Certificati d'analisi (COA)

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Josiane Sancéau et al.
The Journal of biological chemistry, 278(38), 36537-36546 (2003-07-09)
Enhanced expression of (pro)matrix metalloproteinase-9 (MMP-9) is associated with human tumor invasion and/or metastasis. COH cells derived from a highly invasive and metastatic Ewing's sarcoma constitutively express proMMP-9. Transfection of a double stranded RNA that targets the MMP-9 mRNA into
A Bowen et al.
Biochemical and biophysical research communications, 479(4), 793-799 (2016-10-01)
The Wnt signalling pathway in beta-cells has been linked to the development of type 2 diabetes. Investigating the impact of a non-canonical Wnt ligand, Wnt4, on beta-cell function we found that in INS-1 cells, Wnt4 was able to completely block Wnt3a
Ayako Mimata et al.
Cancer science, 102(5), 942-950 (2011-02-01)
Alterations in the E-cadherin gene are associated with sporadic and hereditary diffuse-type gastric cancer. To determine how the loss of function of E-cadherin affects gastric epithelial cell phenotypes, we generated transgenic mice using the Cre-loxP system in which the E-cadherin



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