T7572
Trombina
400-1000 NIH units/mg protein
Sinonimo/i:
Fattore IIa
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
250 units | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 202,00 € |
1000 units | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 617,00 € |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-648-7
MDL number:
Specific activity:
400-1000 NIH units/mg protein
202,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
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Permettici di aiutartiGeneral description
Lyophilized powder containing sucrose, sodium chloride and Tris.
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. [1]
Application
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess an innovative flow cytometric approach for small-size platelet microparticles. [2]
Biochem/physiol Actions
Serin proteasi che scinde in modo selettivo i legami Arg-Gly nel fibrinogeno per formare fibrina e i fibrinopeptidi A e B.
Analysis Note
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Other Notes
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Questo articolo | |||
|---|---|---|---|
| specific activity 400-1000 NIH units/mg protein | specific activity ≥2800 NIH units/mg protein (E1%/280, 18.3) | specific activity ≥1,000 NIH units/mg protein (E1%/280, 18.3) | specific activity 1500-3500 NIH units/mg protein (E1%/280, 18.3) |
| Gene Information human ... F2(2147) | Gene Information human ... F2(2147) | Gene Information human ... F2(2147) | Gene Information human ... F2(2147) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| impurities HIV, hepatitis B and hepatitis C, tested negative | impurities HIV, hepatitis B and hepatitis C, tested negative | impurities HIV and HBsAg, source material tested negative | impurities HIV, hepatitis B and hepatitis C, tested negative |
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