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S6782

Sigma-Aldrich

Superdex®

Superdex®200 Prep Grade

Sinonimo/i:

Size exclusion media, Superdex column

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About This Item

Numero MDL:
MFCD00166816
Codice UNSPSC:
23151817
NACRES:
NA.56

Livello qualitativo

200

Forma fisica

suspension (20% aqueous ethanol)

tecniche

protein array: suitable

Matrice

cross-linked agarose

Dimensione particelle

24-44 μm

Dimensione pori

1-100 kDa MW range (dextrans)
10-600 kDa MW range (proteins)

operating pH range

1-14(short term)
3-12(long term)

Temperatura di conservazione

2-8°C

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Descrizione generale

Combines the high selectivity of a Sephadex matrix with the chemical and physical stability of crosslinked agarose.

Applicazioni

Superdex® is used for protein chromatography, gel filtration chromatography, gel filtration media, resins and separation media. Superdex® has been used for the purification and characterization of a novel laccase from the edible mushroom Hericium coralloides. Superdex® has also been used for the purification and mechanism exploration of a potential human hepatocellular carcinoma inhibitor from Bauhinia purpurea L. seeds.
Optimized for high resolution preparative separations by size exclusion chromatography. Recommended fractionation ranges are listed for globular proteins and dextrans.

Note legali

Superdex is a registered trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Classi di pericolo

Flam. Liq. 3

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

104.0 °F

Punto d’infiammabilità (°C)

40 °C

Certificati d'analisi (COA)

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Superdex® 200 Increase 3.2/300 column L × I.D. 30 cm × 3.2 mm, 8.6 μm particle size

Sigma-Aldrich

GE28-9909-46

Superdex® 200 Increase 3.2/300

HiLoad® 16/600 Superdex® 30 pg Cytiva 28-9893-31, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

Sigma-Aldrich

GE28-9893-31

HiLoad® 16/600 Superdex® 30 pg

Superdex® 30 Prep Grade Cytiva 17-0905-01, pack of 150 mL

GE17-0905-01

Superdex® 30 Prep Grade

Sephadex® G-100 Medium

Sigma-Aldrich

G100120

Sephadex® G-100

Sephacryl® 200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)

Sigma-Aldrich

S200HR

Sephacryl®

J Oh et al.
Human molecular genetics, 9(3), 375-385 (2000-02-03)
Hermansky-Pudlak syndrome (HPS) is a rare autosomal recessive disorder in which oculocutaneous albinism, bleeding tendency and a ceroid-lipofuscin lysosomal storage disease result from defects of multiple cytoplasmic organelles: melanosomes, platelet dense granules and lysosomes. The HPS polypeptide, a 700 amino
Thomas Ouellette et al.
Protein expression and purification, 30(2), 156-166 (2003-07-26)
A recombinant form of human rhIL-7 was overexpressed in Escherichia coli HMS174 (DE3) pLysS under the control of a T7 promoter. The resulting insoluble inclusion bodies were separated from cellular debris by cross-flow filtration and solubilized by homogenization with 6
Mario Jiz et al.
Infection and immunity, 76(7), 3164-3169 (2008-04-23)
Despite effective chemotherapy, schistosomiasis remains a major public health problem in the developing world, with at least 200 million active infections resulting in significant morbidity. Rapid reinfection after treatment, accompanied by extensive residual morbidity, mandates alternative control strategies, including vaccine
J L Van Hove et al.
Biochemistry and molecular biology international, 43(3), 613-623 (1997-11-14)
Large quantities of recombinant acid alpha-glucosidase are needed for in vivo experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A
Partial purification of two proteins (100 kDa and 67 kDa) cooperating in the ATP-dependent inactivation of spinach leaf nitrate reductase.
Spill, D., and Kaiser, W.M.
Planta, 192(2), 183-188 (1994)
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