P7746
Anti-phospho-PAK1/2/3 (pThr423) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
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About This Item
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Forma fisica
buffered aqueous glycerol solution
Reattività contro le specie
human
tecniche
western blot: 1:1,000 using His-tagged wild-type and S139A/T421A double mutant PAK3.
N° accesso UniProt
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
phosphorylation (pThr423)
Informazioni sul gene
human ... PAK1(5058)
Descrizione generale
p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
The antibody detects human PAK1, PAK2 and PAK3, phosphorylated at Thr423. Other species with 100% PAK homology are expected to react.
The antibody detects human PAK1, PAK2 and PAK3, phosphorylated at Thr423. Other species with 100% PAK homology are expected to react.
Immunogeno
synthetic phosphopeptide derived from the region of PAK that contains threonine 423.
Applicazioni
A recommended working concentration of 0.1-1.0 μg/mL is determined by immunoblotting using Histagged wild-type and S139A/T421A double mutant PAK3.
Stato fisico
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificati d'analisi (COA)
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Clemens Hofmann et al.
Journal of cell science, 117(Pt 19), 4343-4354 (2004-08-28)
p21-activated kinases (Paks) are a highly conserved family of enzymes that bind to and are activated by small GTPases of the Cdc42 and Rac families. With the notable exception of plants, nearly all eukaryotes encode one or more Pak genes
Giampiero Porcu et al.
Molecular cancer, 12(1), 88-88 (2013-08-07)
Farnesyltransferase inhibitors (FTIs) are anticancer agents with a spectrum of activity in Ras-dependent and independent tumor cellular and xenograph models. How inhibition of protein farnesylation by FTIs results in reduced cancer cell proliferation is poorly understood due to the multiplicity
Mengwei Zang et al.
The Journal of biological chemistry, 277(6), 4395-4405 (2001-12-26)
Activation of Raf-1 is a complex process in which phosphorylation of Ser(338)-Tyr(341) is a critical step. Previous studies have shown that Pak1/2 is implicated in both Ras-dependent and -independent activation of Raf-1 by phosphorylating Raf Ser(338). The present study explores
Zhou-shen Zhao et al.
The Biochemical journal, 386(Pt 2), 201-214 (2004-11-19)
The Rho GTPases are a family of molecular switches that are critical regulators of signal transduction pathways in eukaryotic cells. They are known principally for their role in regulating the cytoskeleton, and do so by recruiting a variety of downstream
C Chong et al.
The Journal of biological chemistry, 276(20), 17347-17353 (2001-03-30)
The p21-activated kinases (PAKs), in common with many kinases, undergo multiple autophosphorylation events upon interaction with appropriate activators. The Cdc42-induced phosphorylation of PAK serves in part to dissociate the kinase from its partners PIX and Nck. Here we investigate in
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