For testing purposes, this product is reconstituted in cold deionized water at 0.1 - 0,2 un/mL. The solution stability and storage conditions have not been determined. The enzyme may also be dissolved in 0.11 M Tris HCl buffer, pH 8.9 with 0.11 M NaCl and 15 mM MgCl2 according to Worthington Manual, p. 310 (1993). Note that this material is not the raw venom, but the purified enzyme. The enzyme is purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.
P3243
Phosphodiesterase I from Crotalus adamanteus venom
vial of ≥100 units, Purified
Sinonimo/i:
5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase
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About This Item
Prodotti consigliati
Origine biologica
Crotalus adamanteus venom
Livello qualitativo
Stato
solid
Qualità
Purified
Attività specifica
≥20.0 unit/mg solid
Confezionamento
vial of ≥100 units
Temperatura di conservazione
−20°C
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Applicazioni
Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3243 is from Crotalus adamanteus venom and is purified. Product P3243 has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides[1].
Azioni biochim/fisiol
Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.
Definizione di unità
One Unit hydrolyzes one μmole of p-nitrophenyl thymidine-5-phosphate per minute at 25 °C, pH 8.9
Nota sulla preparazione
Purified via the method of Williams, et al.[2] and further treated to inactivate contaminating 5′-nucleotidase activity.
Ricostituzione
For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Action of venom phosphodiesterase on deoxyribonucleic acid.
E J WILLIAMS et al.
The Journal of biological chemistry, 236, 1130-1134 (1961-04-01)
Valérie de Crécy-Lagard et al.
Molecular biology and evolution, 27(9), 2062-2077 (2010-04-13)
Wyosine (imG) and its derivatives such as wybutosine (yW) are found at position 37 of phenylalanine-specific transfer RNA (tRNA(Phe)), 3' adjacent to the anticodon in Eucarya and Archaea. In Saccharomyces cerevisiae, formation of yW requires five enzymes acting in a
Jongwon Lim et al.
ACS omega, 5(23), 14173-14179 (2020-06-23)
The metazoan second messenger 2'3'-cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) is a cyclic dinucleotide (CDN) that induces secretion of type I interferons and activates the immune system and has thus attracted significant interest as a vaccine adjuvant or immunotherapeutic. CDN bisphosphorothioates
Alain R Weber et al.
Nature communications, 7, 10806-10806 (2016-03-05)
Cytosine methylation in CpG dinucleotides is an epigenetic DNA modification dynamically established and maintained by DNA methyltransferases and demethylases. Molecular mechanisms of active DNA demethylation began to surface only recently with the discovery of the 5-methylcytosine (5mC)-directed hydroxylase and base
N Shenoy et al.
Blood cancer journal, 7(7), e587-e587 (2017-07-22)
The Ten Eleven Translocation (TET) enzymes have been found to be mutated in both diffuse large B-cell (DLBCL) and peripheral T-cell (PTCL) lymphomas resulting in DNA hypermethylation. Recent studies in embryonal stem cells showed that ascorbic acid (AA) is a
Protocolli
To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.
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1. If I want to dissolve this venom what I can use distilled water or any buffer? And what is the storage temperature after reconstitution? 2. If I want to increase the efficiency of venom what I can use?
1 risposta-
Utile?
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What buffer can be used to Store PDE I, and is it soluble in water?
1 risposta-
For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL. The solution stability has not been determined.
Utile?
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