A3682
Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Sinonimo/i:
Goat Anti-Mouse IgG (Fab specific)- HRP
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Origine biologica
goat
Coniugato
peroxidase conjugate
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Forma fisica
buffered aqueous solution
Reattività contro le specie
mouse
Non deve reagire con
human, rat
tecniche
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
western blot: 1:80,000-160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per lane)
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Categorie correlate
Descrizione generale
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice possess five classes of immunoglobulins- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody is specific for mouse IgG and the Fab fragment of mouse IgG. The antibody reacts with all mouse IgG subclasses (G1, G2a, G2b and G3) and also with mouse IgA, IgM and IgE. Moreover, the antibody conjugate shows no reactivity with the Fc fragment of mouse IgG or with human and rat IgG.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody is specific for mouse IgG and the Fab fragment of mouse IgG. The antibody reacts with all mouse IgG subclasses (G1, G2a, G2b and G3) and also with mouse IgA, IgM and IgE. Moreover, the antibody conjugate shows no reactivity with the Fc fragment of mouse IgG or with human and rat IgG.
Immunogeno
purified mouse IgG, Fab fragment
Applicazioni
Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.
Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in goat has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemical studies
- dot or immunoblotting
Goat anti-mouse IgG (Fab specific)-peroxidase antibody can be used for western blotting applications. The product can also be used for direct ELISA (1:40,000) and immunohistochemistry (at 1:150, using formalin-fixed, paraffin-embedded sections).
HRP conjugated goat anti-mouse IgG Fab specific was used as the secondary antibody for western blot analysis of bacterial samples of s. equi cells to detect fibrinogen-binding proteins.
Azioni biochim/fisiol
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Stato fisico
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
Nota sulla preparazione
Adsorbed with human IgG and rat serum proteins to reduce background staining with human or rat samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Motore di ricerca dei prodotti.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Astrocytes produce the antiinflammatory and neuroprotective agent hydrogen sulfide
Lee M, et al.
Neurobiology of Aging, 30(10), 1523-1534 (2009)
Dirk Pohlers et al.
Arthritis research & therapy, 9(3), R59-R59 (2007-06-28)
Genome-wide gene expression was comparatively investigated in early-passage rheumatoid arthritis (RA) and osteoarthritis (OA) synovial fibroblasts (SFBs; n = 6 each) using oligonucleotide microarrays; mRNA/protein data were validated by quantitative PCR (qPCR) and western blotting and immunohistochemistry, respectively. Gene set
Moonhee Lee et al.
Journal of neuroinflammation, 13, 32-32 (2016-02-10)
Sodium thiosulfate (STS) is an industrial chemical which has also been approved for the treatment of certain rare medical conditions. These include cyanide poisoning and calciphylaxis in hemodialysis patients with end-stage kidney disease. Here, we investigated the anti-inflammatory activity of
Quercetin, not caffeine, is a major neuroprotective component in coffee
Lee M, et al.
Neurobiology of Aging, 46, 113-123 (2016)
Acidic fibroblast growth factor (FGF) potentiates glial-mediated neurotoxicity by activating FGFR2 IIIb protein
Lee M, et al.
The Journal of Biological Chemistry, 286(48), 41230-41245 (2011)
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.