A3150

Fosfatasi alcalina anti-IgG umane (specifica per la catena γ)−

affinity isolated antibody, buffered aqueous glycerol solution

• Sinonimo/i: Goat Anti-Human IgG (γ-chain specific)−AP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162443
• Conjugate: alkaline phosphatase conjugate
• Clone: polyclonal
• Application: ELISA (d)
• Citations: 15

Proprietà

• biological source: goat
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: human
• technique(s): direct ELISA: 1:7,000-1:21,000
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Descrizione

General description

Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is a glycoprotein antibody that contains two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4.

Immunogen

purified human IgG

Application

Le proteine raccolte da colture di E. coli sono state analizzate su gel SDS-page nel western blotting utilizzando IgG di capra anti-umana (specifica per la catena) coniugata con fosfatasi alcalina a una concentrazione di 1:10 000 per il secondario. Le membrane sono state sviluppate con BCIP/NBT come substrato (Sigma).[1]

Le proteine raccolte da colture di E. coli sono state analizzate su gel SDS-page nel western blotting utilizzando IgG di capra anti-umana (specifica per la catena) coniugata con fosfatasi alcalina a una concentrazione di 1:10 000 per il secondario.[1] L′IgG è stata rilevata nel siero umano con Elisa utilizzando IgG di capra anti-umana (specifica per la catena gamma) coniugata con fosfatasi alcalina diluita 1:5000 in 0,25% BSA/PBS. Il saggio è stato sviluppato con p-nitrofenil fosfato come substrato (1 mg/mL Sigma).[2]

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Goat anti-human IgG (gamma-chain specific)-alkaline phosphatase antibody can be used for ELISA applications using serum samples from allergic and borderline hypertensive patients.

Biochem/physiol Actions

Human IgGs regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat anti-human IgG (gamma-chain specific)-alkaline phosphatase antibody is specific for human IgG when tested against human IgA, IgG, IgM, Bence Jones Kappa, and Lambda myeloma proteins.

IgG1 is most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions. IgG4 is associated with asymptomatic infection. IgG antibody have enormous therapeutic potential and the Fc region aids in the development of therapeutic antibody.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl_2, 15 mM sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Classe di stoccaggio: 10 - Combustible liquids
• wgk: WGK 2