This antibody has not been validated for use in immunohistochemistry on frozen tissue.
A0293
Anti-Human IgG (Fab specific)−Peroxidase antibody produced in goat
affinity isolated antibody
Sinonimo/i:
Anti Human Fab Antibody, Anti Human Fab Antibody - Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat
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Informazioni su questo articolo
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p), WB CL
Citations:
85
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Permettici di aiutartibiological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
human
technique(s)
direct ELISA: 1:40,000, dot blot: 1:100,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:100,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Categorie correlate
1 of 4
Questo articolo | |||
|---|---|---|---|
| technique(s) direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, dot blot: 1:100,000, western blot (chemiluminescent): 1:100,000 | technique(s) direct ELISA: 1:40,000, dot blot: 1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 | technique(s) indirect ELISA: 1:10,000 | technique(s) direct ELISA: 1:40,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot (chemiluminescent): 1:30,000 |
| biological source goat | biological source goat | biological source goat | biological source goat |
| species reactivity human | species reactivity - | species reactivity - | species reactivity - |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate unconjugated | conjugate alkaline phosphatase conjugate |
| antibody form affinity isolated antibody | antibody form IgG fraction of antiserum | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
General description
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,.
Antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and then further purified to remove unconjugated material.
Antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and then further purified to remove unconjugated material.
Immunogen
Fab fragment of human IgG
Application
Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat has been used in:
Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene-diamine substrate solution (Sigma).
- western blot
- direct enzyme linked immunosorbent assay (ELISA) at 1:40,000 dilution for analysis of yeast cultures genetically modified to produced recombinant Fab fragments.
- immunohistochemistry
- dot blot
- immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200)
Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene-diamine substrate solution (Sigma).
Goat anti-human IgG (Fab specific)-peroxidase antibody can be used for direct ELISA (1:40,000), dot blot and immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200) applications.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
Classe di stoccaggio
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Deng Ning et al.
Journal of biochemistry, 134(6), 813-817 (2004-02-11)
Anti-HBs Fab fragment has considerable potential for use in the prevention and treatment of liver diseases by HBV. Here we established a high-level expression system to directly produce anti-HBs Fab fragment in Pichia pastoris. This was achieved by co-integration of
Kristell Lebozec et al.
mAbs, 9(6), 945-958 (2017-06-10)
Glycoprotein VI is a platelet-specific collagen receptor critical for in vivo formation of arterial thrombosis. It is also considered as an attractive target for the development of anti-thrombotic drugs because blocking glycoprotein (GP)VI inhibits platelet aggregation without inducing detrimental effects
A novel approach of prophylaxis to HBV recurrence after liver transplantation
Pan T, et al.
Virology, 382(1), 1-9 (2008)
Daniel Stadlbauer et al.
mSphere, 2(6) (2017-12-16)
Human influenza virus infections with avian subtype H7N9 viruses are a major public health concern and have encouraged the development of effective H7 prepandemic vaccines. In this study, baseline and postvaccination serum samples of individuals aged 18 years and older
Hanieh Khalili et al.
Scientific reports, 6, 36905-36905 (2016-11-23)
Infliximab is an antibody that neutralizes TNF-α and is used principally by systemic administration to treat many inflammatory disorders. We prepared the antibody mimetic Fab-PEG-Fab (FpFinfliximab) for direct intravitreal injection to assess whether such formulations have biological activity and potential
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Contenuto correlato
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| A0293-1ML | 04061833327388 |
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Hola, Se puede usar este anticuerpo para IHC en tejido congelado?
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