49429
Phalloidin–Atto 532
suitable for fluorescence, ≥90.0% (HPLC)
Sinonimo/i:
Atto 532–Phalloidin
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
Scegli un formato
Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
10 nmol | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 464,00 € |
Informazioni su questo articolo
NACRES:
NA.32
UNSPSC Code:
12352108
464,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutartiQuality Segment
assay
≥90.0% (HPLC)
manufacturer/tradename
ATTO-TEC GmbH
λ
in acetonitrile: water (1:9) (with 0.01% trifluoroacetic acid)
UV absorption
λ: 534-540 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
General description
Atto 532 is a novel fluorescent label related to the well-known dye Rhodamine 6G. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, excellent water solubility, and very little triplet formation. After coupling to a substrate Atto 532 Phalloidin carries a net electrical charge of -1. Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10–8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours.
Application
Fluorescent conjugates of phalloidin, rhodamine-phalloidin staining reagents, such as Phalloidin–Atto 532 are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
1 of 1
Questo articolo | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| assay ≥90.0% (HPLC) | assay ≥90.0% (HPLC) | assay ≥90.0% (sum of isomers, HPLC) | assay ≥90% (HPLC) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH |
| UV absorption λ: 534-540 nm Amax | UV absorption λ: 600-606 nm Amax | UV absorption λ: 651- 657 nm Amax | UV absorption λ: 429-435 nm Amax |
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.