MABE337

Anti-Hexim 1 Antibody, clone 10D2.1

clone 10D2.1, from mouse

• Sinonimo/i: Protein HEXIM1, Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein, Hexamethylene bis-acetamide-inducible protein 1, Menage a quatre protein 1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 10D2.1, monoclonal
• Reattività contro le specie: human
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_006451
• N° accesso UniProt: O94992
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... HEXIM1(10614)

Descrizione

Descrizione generale

Hexim1 is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Hexim1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. Hexim1 may also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), Hexim1, Hexim2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. Hexim1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. Hexim1 is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen.

Immunogeno

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human Hexim 1.

Applicazioni

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Hexim 1 in human placenta tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Hexim 1 Antibody, clone 10D2.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Hexim 1.

Qualità

Evaluated by Western Blotting in human placenta tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Hexim 1 in 10 µg of human placenta tissue lysate.

Descrizione del bersaglio

~60 kDa observed

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable