The gelation process for this product does not require pH neutralization by the addition of acid or base. The procedure involves pipetting undiluted collagen into culture dishes, exposing them to ammonia vapor, and rinsing with sterile distilled water multiple times to remove ammonia. The final rinse is replaced with a sterile medium or balanced salt solution with serum, and the dishes are stored at 4°C until use.
08-115
Rat Collagen Type I
from rat tail, liquid, 2-5 mg/mL, suitable for cell culture, used for 3D gel formation
Sinonimo/i:
Collagene di coda di topo, Collagene di tipo I di coda di topo
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Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 100 mg | Disponibile per la spedizione OGGIdaMerck S.p.A | 352,00 € |
Informazioni su questo articolo
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
rat
Form:
liquid, solution
Technique(s):
cell culture | mammalian: suitable (and for 3D proliferation assays.)
Concentration:
100 mg
Servizio Tecnico
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Permettici di aiutartiNome del prodotto
Collagen Type I, rat tail, 100 mg, solution, BioReagent, suitable for cell culture
biological source
rat
Quality Level
form
liquid, solution
manufacturer/tradename
Upstate®
concentration
100 mg
technique(s)
cell culture | mammalian: suitable (and for 3D proliferation assays.)
input
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Gene Information
rat ... COL1A1(29393)
General description
In humans, type I collagen is the most abundant and broadly expressed collagen. This heterotrimer contains two alpha 1 (I) chains and one alpha 2 (I) chain.[1] αα[2] It has repeating Gly-Xaa-Yaa tri-peptides motifs. The collagen alpha-1(I) chain protein is a member of the collagen complex superfamily.[1] COL1A1 has a von Willebrand factor type C (vWFC) domain, a collagen triple-helix repeat, and a fibrillar collagen C-terminal domain (COLF).[2]
Biochem/physiol Actions
Physical form
0.02N acetic acid, pH 3.67
Preparation Note
1 anno a 4°C
Analysis Note
Routinely evaluated by testing for uniform gelation and for attachment of and spreading of PC-12 rat cells
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Salvo diversa indicazione nel nostro catalogo o in altra documentazione fornita dall′azienda insieme al prodotto, i nostri prodotti sono destinati esclusivamente a scopi di ricerca e non devono essere utilizzati per altre finalità, inclusi a titolo esemplificativo ma non esaustivo, fini commerciali non autorizzati, applicazioni diagnostiche in vitro, usi terapeutici ex vivo o in vivo o qualsiasi altro tipo di assunzione o applicazione rivolta agli esseri umani o agli animali.
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Questo articolo | |||
|---|---|---|---|
| biological source rat | biological source rat tail | biological source human skin | biological source rabbit |
| technique(s) cell culture | mammalian: suitable (and for 3D proliferation assays.) | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) ELISA: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable |
| concentration 100 mg | concentration - | concentration - | concentration - |
| Quality Level 100 | Quality Level - | Quality Level 100 | Quality Level 100 |
| form liquid, solution | form lyophilized (clear, colorless solution after reconstitution) | form lyophilized | form - |
| shipped in wet ice | shipped in - | shipped in ambient | shipped in dry ice |
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Classe di stoccaggio
12 - Non Combustible Liquids
wgk
WGK 1
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Protocolli
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
Articoli
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Contenuto correlato
C Fink et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(5), 669-679 (2000-04-01)
To examine the influence of chronic mechanical stretch on functional behavior of cardiac myocytes, we reconstituted embryonic chick or neonatal rat cardiac myocytes to a 3-dimensional engineered heart tissue (EHT) by mixing freshly isolated cells with neutralized collagen I and
J J Tomasek et al.
The Journal of biological chemistry, 272(11), 7482-7487 (1997-03-14)
Gelatinase A (GL-A) is a matrix metalloproteinase (MMP) involved in both connective tissue remodeling and tumor invasion. GL-A activation is mediated by a membrane-type MMP (MT-MMP) that cleaves the GL-A propeptide. In this study, we examined the role of the
H Uyttendaele et al.
Developmental biology, 196(2), 204-217 (1998-05-13)
Elongation and branching of epithelial ducts is a crucial event during the development of the mammary gland. Branching morphogenesis of the mouse mammary epithelial TAC-2 cell line was used as an assay to examine the role of Wnt, HGF, TGF-beta
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| 08-115 | 08436037123627 |
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Does this product require pH neutralization by addition of acid or base for gelation?
1 risposta-
Utile?
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Hi team: I hope this finds you well. May I know the concentration of collagen type I , rat tail 08-115? Much appreciated. Kind regards, Ovi
1 risposta-
The concentration is provided in lot specific Certificates of Analysis, typically within the range of 3 to 5 mg/ml.
Utile?
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Accidentally, I opened the collagen out of the flow chamber. Should I filter it or autoclave it to ensure the sterile conditions?
1 risposta-
Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution.
Avoid sterilizing the collagen solution by membrane filtration, as substantial protein loss may occur by this method. The coating of tissue culture plastic dishes may be performed by air-drying. Dried coated dishes may then be sterilized by exposure to UV light in a sterile culture hood or by rinsing with 70% ethanol.Utile?
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Hi, what is the concentration of the collagen?
1 risposta-
The concentration of this product will be listed on the lot-specific Certificate of Analysis. Please see the link below to access a Certificate:
https://www.emdmillipore.com/US/en/product/Collagen-Type-I-rat-tail,MM_NF-08-115#anchor_COAUtile?
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Once diluted in 0.02N acetic acid, does the collagen solution require sterilisation for use in cell culture? If so, which method is the most effective: UV, autoclave or 0.22uM sterile filter (should this be PES or PVDF)?
1 risposta-
This product is supplied as a sterile solution in 0.02 N Acetic acid. The package size is 100 mg, however, the concentration is variable and reported on the lot-specific Certificate of Analysis. The typical range is 4 - 5 mg/mL. Dilutions can be made with sterile PBS. Further sterilization is not required. If necessary, coated slides and coverslips can be sterilized by UV.
Utile?
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