07-428-I
Anti-phospho eNOS Antibody (Ser1177)
from rabbit, purified by affinity chromatography
Sinonimo/i:
Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
human
Reattività contro le specie (prevista in base all’omologia)
bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), porcine (based on 100% sequence homology), sheep (based on 100% sequence homology), canine (based on 100% sequence homology)
tecniche
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
phosphorylation (pSer1177)
Informazioni sul gene
human ... NOS3(4846)
Descrizione generale
NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
Immunogeno
Epitope: Near C-terminus
KLH-conjugated linear peptide corresponding to a region near the C-terminus of human eNOS phosphorylated at Ser1177.
Applicazioni
Anti-phospho eNOS Antibody (Ser1177) is a highly specific rabbit polyclonal antibody, that targets eNOS/NOS III & has been tested in western blotting.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Qualità
Evaluated by Western Blotting in untreated and VEGF treated HUVEC cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected phospho eNOS (Ser1177) in 10 µg of VEGF treated HUVEC cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected phospho eNOS (Ser1177) in 10 µg of VEGF treated HUVEC cell lysate.
Descrizione del bersaglio
~133 kDa observed
Linkage
Replaces: 07-428
Stato fisico
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Frontiers in cardiovascular medicine, 8, 747497-747497 (2021-10-29)
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European journal of applied physiology (2022-10-29)
Endothelial dysfunction is an early and integral event in the development of atherosclerosis and coronary artery disease (CAD). Reduced NO bioavailability, oxidative stress, vasoconstriction, inflammation and senescence are all implicated in endothelial dysfunction. However, there are limited data examining associations
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The Journal of biological chemistry, 299(3), 102921-102921 (2023-01-22)
Circulating fatty acid-binding protein 3 (FABP3) is an effective biomarker of myocardial injury and peripheral artery disease (PAD). The endothelium, which forms the inner most layer of every blood vessel, is exposed to higher levels of FABP3 in PAD or
Andrea Tryfonos et al.
European journal of sport science, 23(4), 561-570 (2022-02-24)
Although evidence demonstrates the fundamental role of shear stress in vascular health, predominantly through the release of nitric oxide (NO), the mechanisms by which endothelial cells (EC)s sense and transduce shear are poorly understood. In cultured ECs tyrosine phosphorylation of
Reetu R Singh et al.
Hypertension (Dallas, Tex. : 1979), 77(4), 1299-1310 (2021-03-02)
[Figure: see text].
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