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Merck
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重要文件

A1908

Sigma-Aldrich

Asialofetuin 来源于胎牛血清

Type II (Sigma designation)

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About This Item

MDL號碼:
MFCD00130561
分類程式碼代碼:
12352202
NACRES:
NA.61
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生物源

bovine

種類

Type II (Sigma designation)

形狀

powder

技術

activity assay: suitable
inhibition assay: suitable

雜質

salt, essentially free
≤1.0% N-acetylneuraminic acid

溶解度

H2O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow

儲存溫度

2-8°C

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應用

Asialofetuin from fetal calf serum is suitable for use:
  • in the assay of the lactose binding activity of the galectin-7 by immunodetection of in situ binding activity to asialofetuin immobilized on nitrocellulose[1]
  • in the inhibition of zona pellucida hardening of mouse oocytes[2]
  • as an acceptor in sialyltransferase enzyme activity assay[3]
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.

生化／生理作用

Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes.[4] It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.[5]
Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain.[6] It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.[7]

準備報告

Enzymatic cleavage using neuraminidase

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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分析證明 (COA)

Lot/Batch Number
0000387280
0000387280
SLCK5437
SLBD4547V
027K7418

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A C Schroeder et al.
Biology of reproduction, 43(5), 891-897 (1990-11-01)
The zona pellucida of mouse oocytes becomes resistant to chymotrypsin digestion, or "hardened", when spontaneous maturation occurs in serum-free medium (De Felici and Siracusa, Gam Res 1982; 6:107). The hardened zona pellucida is refractory to sperm penetration, thus preventing fertilization.
Serum-resistant lipoplexes in the presence of asialofetuin
Liposomes, 2011, 425-434 (2010)
X C Wang et al.
The Journal of biological chemistry, 264(3), 1854-1859 (1989-01-25)
The hepatic acute phase response is accompanied by increased levels of Gal beta 1-4GlcNAc alpha 2,6-sialyltransferase activity in liver and in circulation. Previous studies suggested that cytokines and glucocorticoids mediate the induction of this sialyltransferase activity. In this study the
W M Brown et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 14(11), 749-755 (1992-11-01)
Bovine fetuin, the first fetal protein to be described, has recently been shown to be a species homologue of a well known human plasma protein--alpha 2HS glycoprotein (alpha 2HS). The fetuins are now known to be members of the cystatin
In vitro gene delivery mediated by asialofetuin-appended cationic liposomes associated with gamma-cyclodextrin into hepatocytes
Motoyama K, et al.
Journal of Drug Delivery, 2011 (2011)
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Questions

  1. What is the difference in Type 1 versus Type II asialofetuin?

    1 answer

    1. The difference between the Type I and Type II Asialofetuin products does not represent any kind of biochemical or functional difference. These indicate different methods of production and purification. The Type II option, product A1908, utilizes neuraminadase in an enzymatic cleavage step. The Type I, product A4781, utilizes a proprietary modification of that described in Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).

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