The difference between the Type I and Type II Asialofetuin products does not represent any kind of biochemical or functional difference. These indicate different methods of production and purification. The Type II option, product A1908, utilizes neuraminadase in an enzymatic cleavage step. The Type I, product A4781, utilizes a proprietary modification of that described in Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).
biological source
bovine
Quality Level
type
Type II (Sigma designation)
form
powder
technique(s)
activity assay: suitable, inhibition assay: suitable
impurities
salt, essentially free, ≤1.0% N-acetylneuraminic acid
solubility
H2O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow
storage temp.
2-8°C
Application
Asialofetuin from fetal calf serum is suitable for use:
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.
Biochem/physiol Actions
Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain.[4] It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.[5]
Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.
Preparation Note
Enzymatic cleavage using neuraminidase
1 of 1
本產品 | |||
|---|---|---|---|
| biological source bovine | biological source bovine (calf) serum | biological source bovine serum (fetal) | biological source bovine serum (fetal) |
| technique(s) activity assay: suitable, inhibition assay: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) HPLC: suitable, MALDI-MS: suitable |
| form powder | form powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| impurities salt, essentially free, ≤1.0% N-acetylneuraminic acid | impurities salt, essentially free, ≤0.5% N-acetylneuraminic acid | impurities ≤0.3% free N-acetylneuraminic acid | impurities ~0.2% free N-acetylneuraminic acid |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
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存儲類別/等級
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
A C Schroeder et al.
Biology of reproduction, 43(5), 891-897 (1990-11-01)
The zona pellucida of mouse oocytes becomes resistant to chymotrypsin digestion, or "hardened", when spontaneous maturation occurs in serum-free medium (De Felici and Siracusa, Gam Res 1982; 6:107). The hardened zona pellucida is refractory to sperm penetration, thus preventing fertilization.
W M Brown et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 14(11), 749-755 (1992-11-01)
Bovine fetuin, the first fetal protein to be described, has recently been shown to be a species homologue of a well known human plasma protein--alpha 2HS glycoprotein (alpha 2HS). The fetuins are now known to be members of the cystatin
Serum-resistant lipoplexes in the presence of asialofetuin
Liposomes, 2011, 425-434 (2010)
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What is the difference in Type 1 versus Type II asialofetuin?
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