病毒載體下游處理
相關技術文章
- 我們對用於病毒載體生產的可擴展切向流過濾的概述將提供開發和優化您的下游製程的關鍵考量因素。
- Benzonase® 內切酶能有效去除病毒生產中的核酸雜質，對細胞和基因療法及疫苗至關重要。
- Explore strategies for separating empty and filled capsids in viral vector production. Learn about upstream strategies and how trends in AAV capsid design impact purification.
尋找更多文章
Primary Clarification
對含有病毒載體的粗培養基進行深度過濾或同時進行離心和深度過濾，以去除大的細胞碎片和其他污染物。
二次澄淨
一次澄淨後的澄清培養基再經過其他步驟進一步澄淨。
超濾/重濾
超濾和重濾分別用於濃縮和緩衝液交換。利用尺寸過濾將病毒載體與雜質分離。重過濾可去除鹽分和其他分子，並以所選的緩衝液取代。
層析
至少使用兩種層析方法來純化病毒載體，包括離子交換法、親和法和尺寸排阻法。
病毒過濾
將純化後的病毒載體進行 15 奈米孔隙的奈米過濾，以去除偶發病毒、內毒素和任何其他殘留的雜質和聚集物。
無菌過濾
無菌過濾或減少生物負荷，可在製程中多次使用。飼料流通過 0.4 或 0.2 微米的消毒級過濾器，以去除在處理過程中引入的細菌和其他微生物。
最終填充
將純化和無菌的病毒載體填充到最終的預定容器中。這裡需要仔細考慮配方、包裝材料、儲存條件，以確保穩定性和完整性。
相關資源
- Shear ignorance? Think again: Breaking the perception of shear within viral vector manufacturing
This podcast transcript discusses best practices – and common misconceptions – when establishing process conditions and utilizing different TFF device formats.
- Pellicon® Capsules versus Hollow Fibers for Ultrafiltration/Diafiltration (UF/DF) in Viral Gene Therapy Manufacturing
This application note discusses methodology and experimental results in the performance evaluation of Pellicon® Capsules versus hollow fibers for UF/DF of viral vectors and describes the impact of either filter type on a TFF process.
- /deepweb/assets/sigmaaldrich/marketing/global/documents/352/641/pellicon-capsule-aav-ot11666en-ms.pdf
Part of the linearly scalable Pellicon® family of TFF filters, single-use Pellicon® Capsules and scale-down Pellicon® XL 50 cassettes provide reliability and reproducibility at any stage of your AAV process, from development to manufacturing
- /deepweb/assets/sigmaaldrich/marketing/global/documents/987/212/pellicon-capsule-vgt-tb11669en-ms.pdf
In this technical brief, we discuss use of our new single-use technology for tangential flow filtration (TFF) of viral vectors during the evaluation of two TFF operating control strategies.
- /deepweb/assets/sigmaaldrich/marketing/global/documents/407/540/podcast-empty-full-separation-ot9653en-ms.pdf
This article explains the need for a more nuanced understanding of AAV capture and empty–full separation, and the trends driving innovation in this area.
若要繼續閱讀，請登入或建立帳戶。
還沒有帳戶？