The form of fetuin has not been determined. This material has not been sequenced. The minimum allowable sialic acid content is 0.5%. The molecular weight is approximately 44,200 daltons. The starting material for this product has a molecular weight of approximately 48,400 including an estimated 8.7% sialic acid. According to the UniPro values for each, the Fetuin-B molecular weight is calculated at 42,663 while the Fetuin-A is 38,419.
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一般說明
Asialofetuin is a glycoprotein with three asparagine-linked triantennary complex carbohydrate chains and terminal N-acetylgalactosamine residues.[1]
應用
Asialofetuin from fetal calf serum has been used:
- to quantitate the plant Ricin toxin′s B subunit (RTB) lectin activity in β-phaseolin signal peptide (P)–proinsulin gene (INS)–RTB plants by enzyme-linked immunosorbent assay (ELISA)[2]
- quantitate VP7:RTB fusion protein in transformed potato tissues by ELISA[3]
- as a glycoprotein substrate to measure the receptor-binding activity of recombinant RTB and NSP490–RTB fusion proteins[4]
- to study the nature of the interaction between ferritin and the placenta[5]
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.
生化／生理作用
Asialofetuin exhibits affinity to asialoglycoprotein receptor (ASGP-R) on hepatocytes and uses the receptor to enter the cell. This property allows asialofetuin to be used as a ligand to deliver drugs to hepatocytes and as a competitive inhibitor to ASGP-R. [1]
準備報告
Prepared by a modification of Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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To wich fetuin corresponds your product A4781 (Asialofetuin from fetal calf serum). Is it Protein Fetuin-A ? Uniprot entry: P12763 FETUA_BOVIN or protein Fetuin-B ? Uniprot entry: Q58D62 FETUB_BOVIN Do you have de amino acid sequence?
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Hi, What is the difference in Type 1 versus Type II asialofetuin
1 answer-
The difference between the Type I and Type II Asialofetuin products does not represent any kind of biochemical or functional difference. These indicate different methods of production and purification. The Type II option, product A1908, utilizes neuraminadase in an enzymatic cleavage step. The Type I, product A4781, uses a proprietary modification of that described in Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).
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