親和層析樹脂
親和層析技術通過高度選擇性的生化相互作用來分離複雜的混合物。這種廣泛使用的純化技術能有效地將目標分子與雜質分離。我們的親和樹脂系列專為高通量、高成本效益的純化而設計,從製程開發到大規模製造,一應俱全。
相關資源
- Tool: mAbsizer™ Calculator
mAb Expression, Purification, and Formulation
- PDF: Biochromatography Portfolio Guide
Bulk Resin, Membranes and Prepacked Columns
- PDF: Chromatography Purification Selection Guide
Chromatography product selection guide.
- Data Sheet: Eshmuno® A Resin
Eshmuno® A media is a rigid, high-capacity, acid and alkalineresistant Protein A affinity chromatography media for the purification of Fc-containing proteins, including but not limited to monoclonal antibodies.
- Data Sheet: Eshmuno® P anti-A and anti-B Resins
High performance, acid and alkaline resistant affinity chromatography resins designed for the removal of anti-A and anti-B antibodies from plasma-derived immunoglobulin (Ig).
- Data Sheet: Fractogel®Chromatography Resin
Fractogel® resins consist of synthetic methacrylate based polymeric beads providing excellent pressure stability resulting in high flow rates. Depending on your purification application, we can offer resin sized M-type beads with a particle size of 40–90 μm and small S-type beads with a particle size in the range of 20–40 μm.
- Application Note: Using Ethylene Glycol Emprove® Evolve to increase Eshmuno®
Sodium hydroxide (NaOH) is a standard cleaning agent for protein A chromatography resins. Typically, a concentration of 0.1 mol/L NaOH is used. The inclusion of 20% ethylene glycol in a 0.1 mol/L NaOH cleaning solution increases the lifetime of Eshmuno® A columns significantly.
- Application Note: Detection and Quantification of Leached Protein A from Eshmuno® A Resin Using Cygnus F400 Protein A ELISA Kit
Cygnus Technologies performed the initial feasibility testing of the Eshmuno® A Protein A ligand in the Cygnus Protein A ELISA Kit (Cat# F400). Upon successful completion of the feasibility testing, Cygnus proceeded with quantification of the assay for use in in-process and drug substance samples using the Eshmuno® A Protein A resin.
- User Guide: MiniChrom® Pre-Packed Columns
The MiniChrom® columns are prepacked chromatography columns designed for laboratory-scale process development evaluations. When used for biomolecule separations, the results achieved with MiniChrom® columns are typically similar to those obtained using traditional laboratory-scale columns from different vendors.
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