病毒載體上游處理
優化基因治療生產流程的上游部分，可最大限度地提高病毒載體滴度，為成功生產奠定基礎。然而，如果沒有經過驗證的範本，製程開發團隊就必須評估病毒載體生產的各個方面，包括
為了將這些製程投入的變異性和風險降到最低，基因治療製造商可以與經驗豐富、在所有方面都具有專業知識的技術合作夥伴合作。a href="/TW/zh/products/pharma-and-biopharma-manufacturing/bioprocessing-cell-culture/viral-vector-upstream-platforms">病毒載體生產以及相關產品和服務的經驗豐富的技術合作夥伴合作。
相關技術文章
- See how the Sf9 rhabdovirus-free cell line was developed and how we’ve developed a companion chemically-defined insect cell media for protein and viral expression.
- Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.
- A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.
- To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.
- Read our white paper on the development of a novel cell culture medium for AAV production with multiple HEK293 lineages and process optimization.
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Thaw and Expand Cells and Seeds
從工作細胞庫或母細胞庫解凍的細胞要解凍和擴大，以增加其數量，為後續的處理步驟做準備。
細胞生長與病毒製造
細胞在生物反應器或其他受控環境中，在特定條件下生長，以最大化病毒製造。
細胞裂解
在 AAV 生產中，宿主細胞會被化學或機械方法裂解，以便病毒可以釋放到培養基。
DNA消化
不需要的全細胞和質粒 DNA 會被核酸酶消化。
相關技術資源
- Product Guide: Upstream Chemicals for Bioprocessing
We offer a broad portfolio of raw materials and chemical components suitable for cell culture media formulations and upstream applications used in biopharmaceutical production.
- White Paper: Application of the Sf-Rhabdovirus-Negative (Sf-RVN®) Platform for AAV Production
The Sf-RVN® Platform is a two-part system comprising a proven Sf-9 cell line devoid of Sf-rhabdovirus (the Sf-RVN® cell line) and a chemically defined medium engineered for the cell line, the EX-CELL® CD Insect Cell Medium.
- Brochure: Gene Therapy Capabilities for AAV and Lentivirus Production
Our products and services for AAV, Lentivirus and other viral vectors provide solutions to your most challenging pain points around process development, speed, manufacturing and regulatory guidelines.
- Transcript: Exploring the Potential of Insect Cell Lines for Efficient AAV Production
Interview transcript with GTRI and their findings of using the Sf-RVN® Platform.
相關影片
Benzonase® 核酸酶能有效地將 DNA 和 RNA 消化成小於 5 個碱基對的片段。
用於改善基因治療和疫苗的安全性。
從擴大規模到克服法規障礙以及快速生產。
從一開始就實施平台流程，成功擴大您的基因治療生產規模。
相關網路研討會
用於基因治療的 AAV 病毒載體的製作會遇到獨特的上游挑戰。了解我們的 HEK 平台如何解決這些挑戰。
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