推薦產品
形狀
buffered aqueous solution
分子量
size 4361 bp
菌種選擇
ampicillin
複製起點
BR322 (15 copies)
肽切割
no cleavage
報告基因
none
運輸包裝
ambient
儲存溫度
−20°C
一般說明
该质粒含有 BR322（低拷贝数）复制起点，并为每个细菌细胞提供约 20 个质粒拷贝。它还包含用于选择转染哺乳动物细胞的MCS和Kan 抗性的哺乳动物CMV启动子上游。低拷贝数质粒对非常大的转基因或对细菌细胞有毒性的转基因是有用的。
启动子表达水平：
启动子表达水平：
應用
BR322 信息： BR322 是从大肠杆菌中分离得到的野生型质粒。其含有低拷贝来源，允许质粒以每个细胞约 10-100 个质粒拷贝的频率维持。通常每个细胞的这一数字接近 30-40个拷贝。最常见的克隆质粒含有该质粒中称为pUC的复制起点的衍生物。pUC 的起源是通过去除 Rep 阻遏蛋白形成的。阻遏蛋白通常在大肠杆菌中调节质粒拷贝数，也在复制起始点本身进行单点突变。点突变是复制起点之间最重要的差异。该质粒可作为通用低拷贝克隆载体。
序列
欲查看本品的序列信息，请访问 产品页面。
分析報告
欲查看本品的分析证书，请访问 www.oxgene.com
相關產品
產品號碼
描述
訂價
儲存類別代碼
12 - Non Combustible Liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual
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