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MOD50

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

同義詞:

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

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關於此項目

UNSPSC Code:
41116158
EC Number:
231-548-0
NACRES:
NA.54
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Quality Segment

200

usage

sufficient for 50 reactions

storage temp.

20-25°C

General description

印记®DNA修饰试剂盒包含完成亚硫酸氢盐转化和随后纯化DNA样本所需的所有试剂。DNA经化学变性，使亚硫酸氢盐试剂与单链DNA特异性反应，从而使胞嘧啶脱氨基并产生尿嘧啶残基。转化的DNA 适用于多种下游应用，包括甲基化特异性PCR、甲基化测序、焦磷酸测序以及甲基化微阵列。

Application

采用印迹®DNA修饰试剂盒进行DNA的亚硫酸氢修改。

Features and Benefits

  • 只需要50微微克的DNA或20个细胞
  • 整个流程用时不到2小时
  • 转化率大于99%
  • 降解率极低
  • 便捷一步协议选项
  • 一致且可重复的亚硫酸氢钠转换
  • 可用于基因组、内切核酸酶消化和FFPE DNA

Preparation Note

所有组分均可在室温下储存。每瓶DNA修饰粉末足以进行10次DNA修饰。溶解后，溶液可在-20°C下避光保存一周。使用前，必须在室温下解冻冷冻溶液并涡旋2分钟。

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

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本產品
CHP1RIPSEQXE
Imprint® DNA Modification Kit For bisulfite DNA conversion & purification

Sigma-Aldrich

MOD50

Imprint® DNA Modification Kit

Imprint® Chromatin Immunoprecipitation Kit Complete ChIP reaction in 6 hours in flexible strip well format

Sigma-Aldrich

CHP1

Imprint® Chromatin Immunoprecipitation Kit

Imprint® RNA Immunoprecipitation Kit High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA

Sigma-Aldrich

RIP

Imprint® RNA Immunoprecipitation Kit

SeqPlex DNA Amplification Kit For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

Sigma-Aldrich

SEQXE

SeqPlex DNA Amplification Kit

usage

sufficient for 50 reactions

usage

-

usage

-

usage

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

20-25°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

套裝中的組件也可單獨購買

產品號碼
描述
SDS & 訂價
  • T3566
    Clear-view™ Snap-Cap microtubes, size 1.5 mL, natural
    SDS

signalword

Danger

supp_hazards

EUH031

存儲類別/等級

8B - Non-combustible corrosive hazardous materials

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000333642
0000333642
SLCQ0447
SLCD4557
SLCD4559

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條款

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR 資料分析

染色質免疫共沉淀 qPCR 用於研究不同條件下的基因調控。

文章

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

相關內容

Imprint ® DNA Modification Kit (MOD50) Protocol

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

Questions

1–6 of 6 Questions  

  1. Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?

    1 answer

    1. Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.

      Helpful?

  4. How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. This kit can be used for 50 reactions.

      Helpful?

  5. Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?

    1 answer

    1. Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment

      Helpful?

  6. Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.

      Helpful?

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