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MOD50

Sigma-Aldrich

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

同義詞:

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

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About This Item

EC號碼:
231-548-0
分類程式碼代碼:
41116158
NACRES:
NA.54
暫時無法取得訂價和供貨情況

用途

sufficient for 50 reactions

儲存溫度

20-25°C

相關類別

一般說明

印记®DNA修饰试剂盒包含完成亚硫酸氢盐转化和随后纯化DNA样本所需的所有试剂。DNA经化学变性，使亚硫酸氢盐试剂与单链DNA特异性反应，从而使胞嘧啶脱氨基并产生尿嘧啶残基。转化的DNA 适用于多种下游应用，包括甲基化特异性PCR、甲基化测序、焦磷酸测序以及甲基化微阵列。

應用

采用印迹®DNA修饰试剂盒进行DNA的亚硫酸氢修改。[1][2][3]

特點和優勢

  • 只需要50微微克的DNA或20个细胞
  • 整个流程用时不到2小时
  • 转化率大于99%
  • 降解率极低
  • 便捷一步协议选项
  • 一致且可重复的亚硫酸氢钠转换
  • 可用于基因组、内切核酸酶消化和FFPE DNA

儲存和穩定性

所有组分均可在室温下储存。每瓶DNA修饰粉末足以进行10次DNA修饰。溶解后，溶液可在-20°C下避光保存一周。使用前，必须在室温下解冻冷冻溶液并涡旋2分钟。

法律資訊

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, naturalSDS

象形圖

CorrosionExclamation mark
GHS05,GHS07

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

安全危害

EUH031

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000333642
0000333642
SLCQ0447
SLCD4557
SLCD4559

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Nelly Olova et al.
Genome biology, 19(1), 33-33 (2018-03-17)
Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available kits, polymerases and bisulfite conversion protocols. Although some steps in the procedure, such
Elena Ivanova et al.
Clinical epigenetics, 12(1), 64-64 (2020-05-13)
Preimplantation embryos experience profound resetting of epigenetic information inherited from the gametes. Genome-wide analysis at single-base resolution has shown similarities but also species differences between human and mouse preimplantation embryos in DNA methylation patterns and reprogramming. Here, we have extended
David E Condon et al.
BMC bioinformatics, 19(1), 31-31 (2018-02-07)
Identification of differentially methylated regions (DMRs) is the initial step towards the study of DNA methylation-mediated gene regulation. Previous approaches to call DMRs suffer from false prediction, use extreme resources, and/or require library installation and input conversion. We developed a
Hwan Young Lee et al.
International journal of legal medicine, 126(1), 55-62 (2011-06-01)
DNA analysis of various body fluid stains at crime scenes facilitates the identification of individuals but does not currently determine the type and origin of the biological material. Recent advances in whole genome epigenetic analysis indicate that chromosome pieces called
Histone H3 Lysine 36 Trimethylation Is Established over the Xist Promoter by Antisense Tsix Transcription and Contributes to Repressing Xist Expression
Ohhata T, et al.
Molecular and Cellular Biology null
查看所有相關論文

文章

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

條款

ChIP-qPCR 資料分析

染色質免疫共沉淀 qPCR 用於研究不同條件下的基因調控。

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

相關內容

Imprint ® DNA Modification Kit (MOD50) Protocol

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

Questions

1–6 of 6 Questions  

  1. Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.

      Helpful?

  2. What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.

      Helpful?

  3. How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?

    1 answer

    1. This kit can be used for 50 reactions.

      Helpful?

  4. Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?

    1 answer

    1. Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.

      Helpful?

  5. Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?

    1 answer

    1. Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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