F9012

抗-山羊IgG（全分子）–FITC 兔抗

IgG fraction of antiserum, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00162348
• Conjugate: FITC conjugate
• Clone: polyclonal
• Application: IF, IHC (p)
• Citations: 8

屬性

• biological source: rabbit
• conjugate: FITC conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160, indirect immunofluorescence: 1:200
• storage temp.: −20°C
• target post-translational modification: unmodified

描述

General description

The immunoglobulin G (IgG) is a major class of immunoglobulin and is highly conserved. IgG constitutes to 10−20% of the plasma proteins. This class of immunoglobulins are glycoproteins containing 82−96% protein and the remaining, carbohydrate. It exists in four subclasses namely, IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains containing two heavy and two light chains, linked by disulfide bonds.

Application

Anti-Goat IgG (whole molecule)-FITC antibody produced in rabbit has been used for immunocytochemistry of human oocytes and preimplantation embryos.

It has also been used in enzyme linked immunosorbent assay (ELISA) and confocal microscopy.[1]

Biochem/physiol Actions

Deficiency of a particular IgG subclass, may increase the susceptibility towards certain pathogens.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti- Goat IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

The antiserum is determined to be immunospecific for goat IgG.

Physical form

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 存儲類別/等級: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)