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U0880

Uricase from Candida sp.

recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

Uricase from Candida sp.

Sinonimo/i:

Urate: oxygen oxoreductase

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Taglio della confezioneSKUDisponibilitàPrezzo
250 units
Per conoscere la disponibilità, visualizza il carrello
CHF 156.00
1000 units
Per conoscere la disponibilità, visualizza il carrello
CHF 463.00

Informazioni su questo articolo

Numero CAS:
9002-12-4
UNSPSC Code:
12352204
PubChem Substance ID:
24900619
NACRES:
NA.54
Numero CE:
1.7.3.3 (BRENDA, IUBMB)
MDL number:
MFCD00082126
Specific activity:
≥2 units/mg solid
Recombinant:
expressed in E. coli

CHF 156.00

Per conoscere la disponibilità, visualizza il carrello
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recombinant

expressed in E. coli

Quality Segment

200

form

lyophilized powder

specific activity

≥2 units/mg solid

mol wt

35 kDa by SDS-PAGE

storage temp.

−20°C

SMILES string

[P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

General description

Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment. [1]
Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

Application

Uricase from Candida sp. has been used:
  • to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
  • to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
  • for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Biochem/physiol Actions

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

Physical form

Powder; contains citrate and sucrose

Other Notes

One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.

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U7128U150094281
Uricase from Candida sp. recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

Sigma-Aldrich

U0880

Uricase from Candida sp.

Uricase from Arthrobacter globiformis lyophilized powder, 15-30 units/mg protein (biuret)

Sigma-Aldrich

U7128

Uricase from Arthrobacter globiformis

Urease from Canavalia ensiformis (Jack bean) Type III, powder, 15,000-50,000 units/g solid

Sigma-Aldrich

U1500

Urease from Canavalia ensiformis (Jack bean)

Urease from Canavalia ensiformis (Jack bean) powder, white, ~8 U/mg

Sigma-Aldrich

94281

Urease from Canavalia ensiformis (Jack bean)

specific activity

≥2 units/mg solid

specific activity

15-30 units/mg protein (biuret)

specific activity

15,000-50,000 units/g solid

specific activity

~8 U/mg

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

lyophilized powder

form

lyophilized powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

mol wt

35 kDa by SDS-PAGE

mol wt

tetramer ~32 kDa

mol wt

~544620 Da

mol wt

Mr ~480000

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

100

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
0000559907
0000536013
0000536012
0000559907
0000536013

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Numero articolo commerciale globale

SKUGTIN
U0880-250UN04061837403859
U0880-1000UN04061837403842
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Questions

1–2 of 2 Questions  

  1. How can a solution in unit/mL be prepared using product number U0880, with a pack size of 250 units and SKU U0880-250UN, considering that the uricase activity is greater than 2 units/mg solid? What is the procedure for preparing a 10 unit/mL solution from this?

    1 answer

    1. You can use the calculator provided at https://www.physiologyweb.com/calculators/units_per_volume_solution_concentration_calculator.html

      Based on a specific activity of 5 units/mg, the 250 unit pack size is equivalent to 50 mg in mass. To achieve a 10 units/mL solution from this, the 50 mg would need to be dissolved in 25 mL of solvent (e.g. PBS).

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  2. What is the amino acid sequence of this enzyme?

    1 answer

    1. The sequence is proprietary.

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