The product is a native protien from Arthrobacter globiformis. This material has not been sequenced. However, this information is published at UniProt.
Please see the link below to review:
https://www.uniprot.org/uniprot/D0VWQ1
U7128
Uricase from Arthrobacter globiformis
lyophilized powder, 15-30 units/mg protein (biuret)
Sinonimo/i:
Urate oxidase, Urate:O2 oxidoreductase
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Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 100 units | Per conoscere la disponibilità, visualizza il carrello | CHF 659.00 |
Servizio Tecnico
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Permettici di aiutartiform
lyophilized powder
Quality Level
specific activity
15-30 units/mg protein (biuret)
mol wt
tetramer ~32 kDa
storage temp.
−20°C
SMILES string
[P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]
InChI
1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
InChI key
VPXDHZMLJOJKOX-UHFFFAOYSA-N
General description
Research area: Cell Signalling. Uricase is made of four identical subunits and is a homotetrameric enzyme. It is found as a functional uricase in several organisms including, bacteria, archaea, and eukaryotes. Nonetheless, primates and humans lack a functional uricase enzyme.
Application
Uricase from Arthrobacter globiformis has been used for coating the electrodes in - biosensor fabrication for the detection of uric acid in urine samples. It has also been used for analysing the effect of Zn, Cu, Mg, and Mn ions on uricase.
Biochem/physiol Actions
Arthrobacter globiformis is a major producer of uricase or urate oxidase in poultry manure and litter. Uricase hydrolyzes uric acid and causes ammonia volatilization in poultry manure. In the majority of mammals, uricase converts uric acid to allantoin, a soluble excretion product. Humans lack uricase due to its loss during the evolution of hominids. This increases the risk of humans towards gout, kidney stones, hypertension, and diabetes due to increased circulating levels of uric acid.Uricase is an important enzyme that plays a role in the purine metabolic pathway.
Molecular weight: 4 × ~32 KDa (tetramer)
Isoelectric point: is 4.64
KM value (uric acid as substrate): ~ 0.01 mM
The optimum pH for activity: 8.5-9.5
The optimum pH for stability: 6.0-9.5
Isoelectric point: is 4.64
KM value (uric acid as substrate): ~ 0.01 mM
The optimum pH for activity: 8.5-9.5
The optimum pH for stability: 6.0-9.5
Zn and Cu were shown to inhibit the activity of uricase. [1]
Other Notes
One unit will convert 1.0 μmole of uric acid to allantoin per min at pH 9.0 at 25 °C.
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Questo articolo | |||
|---|---|---|---|
| specific activity 15-30 units/mg protein (biuret) | specific activity ≥2 units/mg solid | specific activity ≥35 units/mg protein (biuret) | specific activity 8-20 units/mg solid |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| mol wt tetramer ~32 kDa | mol wt 35 kDa by SDS-PAGE | mol wt - | mol wt 71 kDa by SDS-PAGE, 83 kDa by gel filtration |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Contenuto correlato
Uric acid: a danger signal from the RNA world that may have a role in the epidemic of obesity, metabolic syndrome, and cardiorenal disease: evolutionary considerations.
Seminars in Nephrology, 31(5), 394-399 (2011)
Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis.
Kanyong P, et al.
Analytical biochemistry, 428(1), 39-43 (2012)
A Therapeutic Uricase with Reduced Immunogenicity Risk and Improved Development Properties
Andrew C N, et al.
PLoS ONE, 11(12) (2016)
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| U7128-100UN | 04061837410086 |
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Hello, I would like to get theprotein sequence of the Arthrobacter globiformis urucase, catalog U7128. Is it a recombinant enzmye made in E. coli, or is it a native prurified protein from the A. globiformis host. Thank you. Guillaume
1 answer-
Helpful?
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