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Merck

S9012

Sulforhodamine B sodium salt

Powder

Sinonimo/i:

Acid red 52; Acid Rhodamine B

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Informazioni su questo articolo

Formula empirica (notazione di Hill):
C27H29N2NaO7S2
Numero CAS:
3520-42-1
Peso molecolare:
580.65
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24899824
EC Number:
222-529-8
MDL number:
MFCD00010180
Colour Index Number:
45100
Beilstein/REAXYS Number:
3886008

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Nome del prodotto

Sulforhodamine B sodium salt, Technical grade

InChI

1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1

InChI key

SXQCTESRRZBPHJ-UHFFFAOYSA-M

SMILES string

[Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1

form

powder

composition

Dye content, ~75%

εmax

≥85000 at 554-560 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Categorie correlate

General description

Sulforhodamine B (also known as SRB, kiton rhodamine B, lissamine rhodamine B, or sulphorhodamine B) is a bright-pink anionic, aminoxanthene dye carrying two sulfonate substituents.

Application

Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.

It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.

It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
SHBT3820
SHBR6190
SHBR3284
SHBL1004
MKCF2845

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