230162

Sulforhodamine B

Dye content 75 %

• Sinonimo/i: Acid Red 52, Sulforhodamine B monosodium salt
• Formula empirica (notazione di Hill): C₂₇H₂₉N₂NaO₇S₂
• Numero CAS: 3520-42-1
• Peso molecolare: 580.65
• Colour Index: 45100
• Beilstein: 3886008
• Numero CE: 222-529-8
• Numero MDL: MFCD00010180
• Codice UNSPSC: 12171500
• ID PubChem: 24853778
• NACRES: NA.47

Proprietà

• Saggio: ≥73% (HPLC)
• Stato: powder
• Composizione: Dye content, 75%
• tecniche: microbe id | staining: suitable
• Colore: brown to very dark brown; green to very dark green
• Solubilità: methanol: 1 mg/mL
• λmax: 554 nm
• ε (coefficiente d’estinzione): ≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• Stringa SMILE: [Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1
• InChI: 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1; SXQCTESRRZBPHJ-UHFFFAOYSA-M

Descrizione

Descrizione generale

Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.[1]

Applicazioni

Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.[2]

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)