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P1253

Phosvitin from egg yolk

Name: Phosvitin from egg yolk
Brand: SIGMA

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Informazioni su questo articolo

Numero CAS:

9008-96-2

UNSPSC Code:

12352202

NACRES:

NA.81

MDL number:

MFCD00131908

Form:

powder

Biological source:

egg yolk

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Proprietà

biological source

egg yolk

Quality Segment

200

form

powder

storage temp.

−20°C

Descrizione

General description

Phosvitin from egg yolk is a polyanionic phosphoserine rich protein.[1] This phosphoglycoprotein comprises α-helices and β-sheets structural elements, which are poorly organized due to the presence of phosphoserine.[1] It represents about 11% of yolk proteins and is hydrophilic.[2]

Application

Phosvitin from egg yolk has been used:

as a control protein for immobilization on-chip for deep purple protein staining methods for monitoring post-translation modifications[3]
as a substrate for casein kinase I_ε in in vitro casein kinase assay[4]
as a standard in gel filtration chromatography (GFC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantification of phosvitin from egg extracts[5]
for immobilization onto Layer-by-Layer (LbL) for Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) studies[6]

Biochem/physiol Actions

Phosvitin fed to rats by adding egg yolk to their food resulted in decreased absorption of magnesium, iron and calcium but not of phosphorus. [7]

Phosvitin from egg yolk is a calcium chelator[1] and binds to iron present in the yolk.[2] The metal-binding functionality is exploited in mineral-binding bioactive peptide production.[2] It is a nutraceutical and has potential for phosphopeptides production.[2]

Other Notes

A phosophoprotein containing 8-10% phosphorus. Molar N/P ratio approx. 2.7.

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Questo articolo	A9207	C7786	L4754
Sigma-Aldrich P1253 Phosvitin from egg yolk	Sigma-Aldrich A9207 Avidin−Agarose from egg white	Sigma-Aldrich C7786 Conalbumin from chicken egg white	Sigma-Aldrich L4754 3-sn-lisofosfatidiletanolammina
biological source egg yolk	biological source egg white	biological source chicken egg white	biological source egg yolk
form powder	form aqueous glycerol suspension	form powder	form powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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