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B5144

Sigma-Aldrich

Bromelain from pineapple stem

≥4 units/mg protein, (chromatography purified)

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100 UNITS
CHF 373.00
250 UNITS
CHF 730.00

CHF 373.00


Spedizione prevista il29 maggio 2025



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Cambia visualizzazione
100 UNITS
CHF 373.00
250 UNITS
CHF 730.00

About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

CHF 373.00


Spedizione prevista il29 maggio 2025


Stato

lyophilized powder

Livello qualitativo

Attività specifica

(chromatography purified)
≥4 units/mg protein

Composizione

Protein, 30-50% biuret

Solubilità

H2O: soluble 1.0 mg/mL, clear, colorless

Temperatura di conservazione

−20°C

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Applicazioni

Bromelain from pineapple stem has been used as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) zymograms.[1] It has also been used as a Azocasein substrate for the determination of proteolytic activity.[2]
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen [3].

Azioni biochim/fisiol

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Definizione di unità

One unit will release 1.0 μmole of p-nitrophenol from N-α-Z-L-lysine p-nitrophenyl ester per min at pH 4.6 at 25 °C.

Stato fisico

Lyophilized powder containing mannitol and potassium phosphate buffer salts

Nota sulla preparazione

Chromatographically purified
Chromatographically purified. Dissolves in water at 1 mg/mL concentration to form a clear, colorless solution.

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Substrato

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Pittogrammi

Health hazardExclamation mark

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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I clienti hanno visto anche

Slide 1 of 4

1 of 4

Acidic cysteine protease inhibitors from pineapple stem.
R L Heinrikson et al.
Methods in enzymology, 45, 740-751 (1976-01-01)
Ashley G Yow et al.
Scientific reports, 13(1), 11605-11605 (2023-07-19)
Papain (aka C1A) family proteases, including bromelain enzymes, are widespread across the plant kingdom and play critical regulatory functions in protein turnover during development. The proteolytic activity exhibited by papain family proteases has led to their increased usage for a
Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International (2016)
Nora Luniak et al.
Biotechnology progress, 33(1), 54-65 (2016-11-20)
Expression of proteases in heterologous hosts remains an ambitious challenge due to severe problems associated with digestion of host proteins. On the other hand, proteases are broadly used in industrial applications and resemble promising drug candidates. Bromelain is an herbal
Tino Krell et al.
Biotechnology and applied biochemistry, 41(Pt 3), 241-246 (2004-09-21)
Vaccines against poliomyelitis and influenza contain inactivated forms of poliovirus and influenza virus. These antigens are generated on an industrial scale from the purified active viruses that have been analysed in this study by DSC (differential scanning calorimetry). Multiple unfolding

Questions

  1. Good day! I would like to ask if do you have a Bromelain Powder in CDU? We would like to purchase for our research regarding bromelain enzyme.

    1 answer
    1. Currently, there are no Bromelain products tested with casein and reported in CDU.

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