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A9934

Aminopeptidase I from Streptomyces griseus

Name: Aminopeptidase I from <I>Streptomyces griseus</I>
Brand: SIGMA
Price: 262.65 CHF

lyophilized powder, ≥200 units/mg protein

Sinonimo/i:

Leucine Aminopeptidase IV

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Taglio della confezione	SKU	Disponibilità	Prezzo
0.1 mg		Per conoscere la disponibilità, visualizza il carrello	CHF 309.00 CHF 262.65

Informazioni su questo articolo

Numero CAS:

9031-94-1

Numero CE:

3.4.11.22 (BRENDA, IUBMB)

UNSPSC Code:

12352204

EC Number:

232-874-6

NACRES:

NA.54

MDL number:

MFCD00166325

Specific activity:

≥200 units/mg protein

CHF 262.65

Prezzo di listinoCHF 309.00Risparmia il 15%

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Proprietà

form

lyophilized powder

Quality Segment

200

specific activity

≥200 units/mg protein

mol wt

21 kDa by gel filtration, 33 kDa by SDS-PAGE

composition

Protein, 40-60% Lowry

storage temp.

−20°C

Descrizione

General description

Aminopeptidase I from Streptomyces griseus is a thermostable enzyme with Glu131 and Tyr246 as key active site residues.[1]

Application

Aminopeptidase I from Streptomyces griseus has been used:

to test the biochar exposure effect on the enzyme activity
in circular dichroism (CD) spectroscopy studies[2]
as a positive control in p-nitroanilide degradation assay[3]

Biochem/physiol Actions

Aminopeptidase I from S. griseus has a fairly broad specificity, being able to remove the N-terminal residue of most proteins, except where the penultimate residue is an imino acid. It contains two Zn²⁺ binding sites. Aminopeptidase I from S. griseus is inhibited by 1,10-phenanthroline and is activated six-fold by Ca²⁺, which also stabilizes it against heat inactivation. This monomeric zinc metalloprotein has an isoelectric point (pI) of 5.4.

Aminopeptidase I may also be used as a reagent in the assay of endoprotease activities with a synthetic substrate in a two-stage assay. In the first stage, the endoprotease cleaves a peptide, such as Z-Y-X-Leu-p-nitroanilide, with the X, Y, and Z residues being chosen according to the specificity of the endoprotease.

Packaging

Package size based on protein content.

Physical form

Contains calcium acetate

Preparation Note

Reconstitute in 20 mM tricine, pH 8.0, with 0.05% bovine serum albumin. Dilute the enzyme with the reconstitution buffer to 0.15-0.3 U/mL for a working concentration. Solutions should be prepared fresh prior to use.

Other Notes

Endopeptidase contaminant: Not more than: 0.01 U/mg protein (as μmole tyrosine equivalent per min released from casein.)

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0, 25 °C and 3.0 mM substrate concentration.

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Questo articolo	P5147	P6236	P6911
Sigma-Aldrich A9934 Aminopeptidase I from Streptomyces griseus	Sigma-Aldrich P5147 Protease from Streptomyces griseus	Sigma-Aldrich P6236 Pyroglutamate Aminopeptidase from Pyrococcus furiosus	Sigma-Aldrich P6911 Protease from Streptomyces griseus
specific activity ≥200 units/mg protein	specific activity ≥3.5 units/mg solid	specific activity ≥5.0 units/mg protein	specific activity -
form lyophilized powder	form powder	form lyophilized powder	form powder
mol wt 21 kDa by gel filtration, 33 kDa by SDS-PAGE	mol wt -	mol wt 24.072 kDa by amino acid sequence, 28 kDa by SDS-PAGE	mol wt monomer ~20 kDa
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
composition Protein, 40-60% Lowry	composition -	composition -	composition -