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A6559

Sigma-Aldrich

Adenosine 5′-triphosphate disodium salt solution

Crystalline ATP, HPLC purified, aqueous solution for RNA transcription

Sinonimo/i:

ATP

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25 μMOL
CHF 205.00

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25 μMOL
CHF 205.00

About This Item

Numero CAS:
987-65-5
Numero MDL:
MFCD00080339
Codice UNSPSC:
41106305
eCl@ss:
32160414
ID PubChem:
24891106
NACRES:
NA.52

CHF 205.00

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Livello qualitativo

300

Stato

liquid

Concentrazione

100 mM in H2O (adjusted to pH 7 with Trizma®
base)

Attività estranea

DNase, RNase, none detected

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Stringa SMILE

[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O

InChI

1S/C10H16N5O13P3.2Na/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;/q;2*+1/p-2/t4-,6-,7-,10-;;/m1../s1
TTWYZDPBDWHJOR-IDIVVRGQSA-L

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Categorie correlate

Descrizione generale

Adenosine 5′-triphosphate disodium salt solution is suitable for use in molecular biology applications such as kinase reactions or DNA-dependent RNA polymerase transcription.

Applicazioni

Adenosine 5′-triphosphate disodium salt solution has been used:
  • as a standard to evaluate the extracellular ATP release from marginal cells of stria vascular[1]
  • as a component of the internal pipette solution to establish a recording electrode to study neurite stretching[2]
  • in in vitro VPS34 (catalytic subunit of the phosphatidylinositol 3-kinase (PtdIns3K) complex) activity assay[3]
  • as a component of lysis and resealing buffers for ATP studies[4]
  • in spectrophotometric measurement of H+ transport in fluorescein isothiocyanate (FITC)-dextran loaded A549 (adenocarcinomic human alveolar basal epithelial cells) and HeLa cells[5]
  • to induce pyroptosis in lipopolysaccharide treated fibroblast-like synoviocytes (FLSs)[6]

Azioni biochim/fisiol

Adenosine 5′-triphosphate (ATP) is an important energy currency in all living organisms. It possesses high phosphate transfer potential. ATP is involved in several biological processes such as membrane transport, muscle contraction and synthesis, and degradation of biological molecules. It plays a role as an intracellular and extracellular signaling molecule in certain cellular processes such as cell motility, organ development, neurotransmission, and insulin secretion.[7]

Note legali

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000394151
0000366958
0000394151
0000366958
0000274502

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Yahui Huang et al.
Archiv der Pharmazie (2018-06-29)
Inhibition of spleen tyrosine kinase (Syk) is a promising strategy for the treatment of various allergic and autoimmune disorders such as asthma, rheumatoid arthritis, and allergic rhinitis. Previously, a Syk inhibitor with novel indazole scaffold was discovered by structure-based virtual
James Franklin Tucker et al.
PLoS genetics, 12(2), e1005873-e1005873 (2016-02-20)
Epigenetic gene silencing plays a critical role in regulating gene expression and contributes to organismal development and cell fate acquisition in eukaryotes. In fission yeast, Schizosaccharomyces pombe, heterochromatin-associated gene silencing is known to be mediated by RNA processing pathways including
Cooper GM
The Cell: A Molecular Approach (2000)
Shaojian Chen et al.
Cell cycle (Georgetown, Tex.), 20(12), 1107-1121 (2021-06-08)
Studies have found that cell pyroptosis is involved in the occurrence and development of rheumatoid arthritis (RA). Hsa_circ_0044235 has been found to be significantly low-expressed in RA patients. The purpose of this research was to reveal the regulatory mechanism of
Identification of small molecule inhibitors of phosphatidylinositol 3-kinase and autophagy.
Farkas T
The Journal of Biological Chemistry, 286(45), 38904-38912 (2011)
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Questions

1–9 of 9 Questions  

  1. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  2. Can this preparation of Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used as an ATP standard?

    1 answer

    1. This preparation of ATP is not recommended for use as an ATP standard. The recommended ATP products for use as a standard in bioluminescence experiments is Product Code FL-AAS (preweighed vials of 1 mg each = 2 micromole).

      Helpful?

  3. What is the binding affinity of ATP (Product No. A6559) for Mg++ and other metal ions?

    1 answer

    1. ATP has intrinsic metal binding affinity. The binding constant for various metals are (given as per mole): Mg++, 9554; Na+, 13; Ca++, 3722; K+, 8; Sr++, 1381; Li+, 25.  The ability of ATP to chelate magnesium results in effective Mg++ ion concentrations lower than the total Mg ion concentration.  For example, in a reaction with 1.5mM Mg++ and 0.2 mM ATP, the effective Mg ion concentration is ~1.3mM.

      Helpful?

  4. Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used in molecular biology applications?

    1 answer

    1. This is a Molecular Biology grade ATP. It is certified free of DNase and RNase. This product is recommended for any typical molecular biology application.

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  5. Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used for in vitro transcription?

    1 answer

    1. The purity of this product should be sufficient for use in T7 RNA polymerase-catalyzed in vitro transcription assay, but we do not test the product for use in that application in our quality control process.

      Helpful?

  6. How long can aqueous ATP solutions like Product No. A6559 be stored?

    1 answer

    1. Aqueous solutions of ATP are stable for months when frozen in solution at -15 °C and for approximately one week at 0 °C.  As with most products, we guarantee the quality of this material for 1 year when stored unopened at the recommended storage temperature (-20°C in this case).

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  7. What is the millimolar extinction coefficient for ATP, Product No. A6559?

    1 answer

    1. The millimolar extinction coefficient (EmM) is 15.4, measured at 259 nm for a sample in 100 mM phosphate buffer, pH 7.0.

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  8. What is meant by 25UMO in the size description of Product A6559, Adenosine 5´-triphosphate disodium salt solution?  What is the product dissolved in?

    1 answer

    1. 25UMO means 25 micromoles.  The 25 UMO size is equivalent to 250 μl of a 100 mM solution. This product is a 100 mM solution in water; the solution pH has been adjusted with Trizma base but contains no additional buffer or salts.

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  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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