233156

Cleland′s Reagent, Molecular Biology Grade

A protective agent for SH groups.

• Sinonimo/i: Cleland’s Reagent, Molecular Biology Grade, 1,4-Dithiothreitol, DTT
• Formula empirica (notazione di Hill): C₄H₁₀O₂S₂
• Numero CAS: 3483-12-3
• Peso molecolare: 154.25
• UNSPSC Code: 12161504
• NACRES: NA.25
• MDL number: MFCD00004877
• Assay: ≥99% (titration)
• Form: powder, solid

Proprietà

• grade: Molecular Biology
• Quality Level: 100
• description: Merck USA index - 14, 3376
• assay: ≥99% (titration)
• form: powder, solid
• reaction suitability: reagent type: reductant
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: water: 15 mg/mL
• cation traces: heavy metals: ≤0.001%
• absorption: ≤0.100 at 280 nm at 100 mM (1 cm), ≤0.500 at 260 nm at 100 mM (1 cm)
• foreign activity: DNases, proteases, RNases, none detected
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: SC[C@H](O)[C@@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• InChI key: VHJLVAABSRFDPM-IMJSIDKUSA-N

Descrizione

General description

Water-soluble protective reagent for sulfhydryl groups [absorbance (100 mM): ≤0.5 at 260 nm; ≤0.1 at 280 nm]. It is capable of maintaining monothiols completely in the reduced state and of reducing disulfides quantitatively. Since it readily permeates cell membranes, it can protect protein sulfhydryls and restore enzyme activity lost by oxidation.

A protective agent for SH groups.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Informazioni sulla sicurezza

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302,H315,H318
• pcodes: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Classe di stoccaggio: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable