05-347
Anti-PCNA Antibody, clone PC10
clone PC10, Upstate®, from mouse
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Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 100 μg | Per conoscere la disponibilità, visualizza il carrello | CHF 402.00 |
Informazioni su questo articolo
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
PC10, monoclonal
Application:
ICC, IHC, WB
Citations:
21
Servizio Tecnico
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Permettici di aiutartibiological source
mouse
Quality Level
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
PC10, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PCNA(5111)
General description
36kDa
Expression of proliferating cell nuclear antigen (PCNA), cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. Anti-PCNA reacts with PCNA at the molecular weight of 36 kDa on western blot.
Immunogen
Recombinant rat PCNA
Application
Detect PCNA with Anti-PCNA Antibody, clone PC10 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC & IHC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Biochem/physiol Actions
PCNA
Physical form
Concentrated culture supernatant
Format: Purified
Protein A purified mouse immunoglobulin in PBS containing 1 mg/mL BSA and 10mM Sodium Azide.
Preparation Note
2 years at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysate from human A431 cells or mouse 3T3/A31 cell lysate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Questo articolo | |||
|---|---|---|---|
| species reactivity vertebrates | species reactivity vertebrates, invertebrates | species reactivity invertebrates, vertebrates | species reactivity human |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| antibody form culture supernatant | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| clone PC10, monoclonal | clone PC10, monoclonal | clone PC10, monoclonal | clone PC10, monoclonal |
| shipped in wet ice | shipped in ambient | shipped in wet ice | shipped in wet ice |
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Classe di stoccaggio
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Xiangyuan Wang et al.
Fetal diagnosis and therapy, 29(4), 315-320 (2011-01-14)
We investigated the patterns of expression of HOXB5, cyclin D1 and proliferating cell nuclear antigen (PCNA) proteins in human congenital cystic adenomatoid malformation (CCAM) to establish the molecular basis of its etiology. Immunohistochemistry was performed on frozen archival specimens of
Mechanism of elongation of primed DNA by DNA polymerase delta, proliferating cell nuclear antigen, and activator 1.
Lee, S H and Hurwitz, J
Proceedings of the National Academy of Sciences of the USA, 87, 5672-5676 (1990)
Proliferating cell nuclear antigen is required for DNA excision repair.
Shivji, K K, et al.
Cell, 69, 367-374 (1992)
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| 05-347 | 04053252627644 |