P6486
EZview™ Red Protein A Affinity Gel
Sinonimo/i:
EZview Affinity Gel, Protein A Affinity Gel
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Forma fisica
liquid
Classi chimiche degli analiti
proteins (Immunoglobulins of various mammalian species)
tecniche
affinity chromatography: suitable
immunoprecipitation (IP): suitable
Capacità
~4 mg binding capacity (rabbit IgG)
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
EZview™ Red Protein A Affinity Gel is a highly visible, red colored Protein A affinity gel. The affinity resin contains Staphylococcus aureus Protein A covalently attached to crosslinked 4% agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue. The immune complexes, bound to the EZview™ Red Protein A Affinity Gel, are recovered by centrifugation. Red dye enhances visibility for more efficient results.
Applicazioni
EZview™ Red Protein A Affinity Gel has been used for purification of proteins in the preparation of immunoprecipitation and chromatin immunoprecipitation (ChIP) assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Caratteristiche e vantaggi
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Stato fisico
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
50% slurry in PBS with 50%Glycerol and 0.0015%
Stoccaggio e stabilità
EZview™ Red Protein A Affinity Gel should be stored in 50% glycerol at -20 °C. This product is stable for at least one year when stored as indicated. Do not freeze in the absence of glycerol.
Note legali
EZview is a trademark of Sigma-Aldrich Co. LLC
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
NF-?B directly regulates ?-arrestin-1 expression and forms a negative feedback circuit in TNF-a?induced cell death
Li J, et al.
Faseb Journal (2018)
Katriina Kahlos et al.
Molecular and cellular biochemistry, 254(1-2), 47-54 (2003-12-17)
We previously reported that exposure to exogenous nitric oxide (NO) causes diminished expression of thioredoxin/thioredoxin reductase, a critical component of the redox system that regulates the functions of redox-sensitive enzymes, receptors, and transcription factors. Here we examined the role of
A laboratory practical illustrating the use of the ChIP-qPCR method in a robust model: Estrogen receptor alpha immunoprecipitation using Mcf-7 culture cells
Lacazette E
BioChemistry: An Indian Journal (2017)
Hsp90 Co-chaperone p23 contributes to dopaminergic mitochondrial stress via stabilization of PHD2: Implications for Parkinson's disease.
Rane A, et al.
Neurotoxicology (2018)
Donna L Cioffi et al.
Circulation research, 97(11), 1164-1172 (2005-10-29)
Store-operated calcium (SOC) entry represents the principal Ca2+ entry pathway into nonexcitable cells. Despite intensive investigation, mechanisms underlying activation of SOC entry have remained elusive. The endothelial ISOC channel is a Ca2+-selective SOC entry channel to which the transient receptor
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.