E3403
EZview™ Red Protein G Affinity Gel
Sinonimo/i:
EZview Affinity Gel, Protein G Affinity Gel
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About This Item
Prodotti consigliati
Forma fisica
liquid
Classi chimiche degli analiti
proteins (Immunoglobulins of various mammalian species)
tecniche
affinity chromatography: suitable
immunoprecipitation (IP): suitable
Capacità
≥8 mg/mL, gel binding capacity (rabbit IgG)
Temperatura di conservazione
−20°C
Categorie correlate
Descrizione generale
EZ view Red Protein G Affinity Gel is Protein G covalently bonded to 4% Agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue, for purification of proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.
Applicazioni
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Caratteristiche e vantaggi
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Stato fisico
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
50% slurry in PBS with 50%Glycerol and 0.0015%
Note legali
EZview is a trademark of Sigma-Aldrich Co. LLC
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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Contenuto correlato
Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.
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