Protein A Sepharose CL-4B exhibits high chemical and mechanical stability, and is stable over the pH range 2-11. It withstands high concentrations of denaturants, such as urea and guanidine hydrochloride. The gel displays high thermal stability but is not autoclavable. Furthermore, the gel can, if handled with care, be used many times.
P3391
Protein A-Sepharose™ from Staphylococcus aureus
lyophilized powder
Sinonimo/i:
Protein A–Agarose
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
Scegli un formato
Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 250 mg | Per conoscere la disponibilità, visualizza il carrello | CHF 270.00 | |
| 1 g | Per conoscere la disponibilità, visualizza il carrello | CHF 795.00 | |
| 1.5 g | Per conoscere la disponibilità, visualizza il carrello | CHF 1’010.00 | |
| 5 g | Per conoscere la disponibilità, visualizza il carrello | CHF 2’640.00 |
Informazioni su questo articolo
Form:
lyophilized powder
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutartiform
lyophilized powder
Quality Segment
packaging
glass bottle of 1 g, glass bottle of 1.5 g, glass bottle of 250 mg, glass bottle of 5 g
storage condition
do not freeze
extent of labeling
3 mg per mL
technique(s)
affinity chromatography: suitable
color
white to gray
matrix
Sepharose CL-4B
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
≥16 mg/mL binding capacity (human IgG)
solubility
water: 1 g/L at 20 °C
suitability
conforms to structure for swelling capacity (1g swells to 4 to 5 mL)
storage temp.
2-8°C
General description
Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.[1]
Application
Protein A-Sepharose was used:
- for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.[2]
- in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.[3]
- as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.[4]
- in immunoprecipitation of [32P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.[5]
Protein A-Sepharose™ is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Physical form
Supplied as lyophilized powder stabilized with lactose and dextran.
Preparation Note
Swelling: 1 g swells to approx. 4 ml.
Legal Information
Sepharose is a trademark of Cytiva
1 of 1
Questo articolo | |||
|---|---|---|---|
| technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable | technique(s) ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) - |
| suitability conforms to structure for swelling capacity (1g swells to 4 to 5 mL) | suitability - | suitability - | suitability - |
| form lyophilized powder | form aqueous ethanol suspension | form powder | form cell suspension |
| storage condition do not freeze | storage condition - | storage condition - | storage condition - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility water: 1 g/L at 20 °C | solubility - | solubility H2O: 1 mg/mL, clear, colorless | solubility - |
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Protocolli
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
Articoli
Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).
Contenuto correlato
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
V Ossipow et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(17), 8219-8223 (1993-09-01)
The CCAAT/enhancer-binding protein (C/EBP) alpha is a leucine zipper protein that is preferentially expressed in certain cell types, such as adipocytes and hepatocytes. Here we show that C/EBP alpha mRNA is translated into two major proteins, C/EBP-42 and C/EBP-30, that
Lionel Gissot et al.
Plant physiology, 142(3), 931-944 (2006-10-10)
The sucrose nonfermenting-1 protein kinase (SNF1)/AMP-activated protein kinase subfamily plays a central role in metabolic responses to nutritional and environmental stresses. In yeast (Saccharomyces cerevisiae) and mammals, the beta- and gamma-noncatalytic subunits are implicated in substrate specificity and subcellular localization
M Iezzi et al.
Molecular endocrinology (Baltimore, Md.), 13(2), 202-212 (1999-02-11)
Insulin-secreting cells express four GTPases of the Rab3 family. After separation of extracts of INS-1 cells on a sucrose density gradient, the bulk of the A, B, and C isoforms was recovered in the fractions enriched in insulin-containing secretory granules.
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| P3391-5G | 04061834367819 |
| P3391-250MG | 04061835512874 |
| P3391-1G | 04061835573851 |
| P3391-1.5G | 04061834367802 |
-
What can P3391, Protein A-Sepharose® resin tolerate?
1 answer-
Helpful?
-
-
How do I swell P3391, Protein A-Sepharose® resin?
1 answer-
The lyophilized product should be swollen in buffer A for 30 minutes or longer at room temperature. Do not stir with any kind of mechanical stirrer. One gram of powder typically swells to 3-4 mL of the hydrated gel. This, along with other useful information (such as the formulation of buffer A) can be found on the product information sheet (under Documents, above).
Helpful?
-
-
What is the binding capacity of Product P3391, Protein A-Sepharose®, resin for my immunoglobulins?
1 answer-
A table is available on the product information sheet (under Documents, above).
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
How can I store the hydrated P3391, Protein A-Sepharose® resin?
1 answer-
Suspensions stored in the refrigerator in the presence of a suitable bacteriostatic agent, such as 20% ETOH, retain IgG binding activity. Another storage option is to put the resin into phosphate buffer saline, pH 7 with about 0.01% preservative (thimerosal or sodium azide).
Helpful?
-
-
How do I clean Product P3391, Protein A-Sepharose®, resin?
1 answer-
It may be possible to clean the resin by washing it with 10-20 volumes of 100 mM Tris or borate buffer, pH8.5, containing 0.5-2.0 M NaCl, followed by 10-20 volumes of 100 mM acetate buffer, pH 4.0, containing0.5-2.0 M NaCl. Re equilibrate the resin with 20 volumes of buffer A.
Helpful?
-