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P1034

Sigma-Aldrich

Phenyl isothiocyanate

Sigma Grade, 8.36 M, suitable for solid phase protein sequencing analysis, ≥99% (GC), liquid

Sinonimo/i:

PITC

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About This Item

Formula condensata:
C6H5NCS
Numero CAS:
103-72-0
Peso molecolare:
135.19
Beilstein:
471392
Numero CE:
203-138-1
Numero MDL:
MFCD00004798
Codice UNSPSC:
12352118
ID PubChem:
24898144
NACRES:
NA.31

Grado

Sigma Grade

Livello qualitativo

100

Saggio

≥99% (GC)

Forma fisica

liquid

Concentrazione

8.36 M

Indice di rifrazione

n20/D 1.6515 (lit.)

P. eboll.

218 °C (lit.)

Punto di fusione

−21 °C (lit.)

Densità

1.132 g/mL at 20 °C (lit.)

Compatibilità

suitable for solid phase protein sequencing analysis

Temperatura di conservazione

2-8°C

Stringa SMILE

S=C=Nc1ccccc1

InChI

1S/C7H5NS/c9-6-8-7-4-2-1-3-5-7/h1-5H
QKFJKGMPGYROCL-UHFFFAOYSA-N

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Categorie correlate

Descrizione generale

Phenyl isothiocyanate belongs to the class of aromatic compounds with isothiocyanate groups. It is typically used as a precolumn derivatization agent for the formation of phenylthiocarbamyl amino acids for their subsequent determination using chromatography techniques.

Applicazioni

Phenyl isothiocyanate may be used in the polypeptide and protein sequencing at the subpicomole and subnanomole levels, respectively, by applying automated Edman chemistry followed by the analysis using high-performance liquid chromatography (HPLC) technique.
Derivatizing reagent for primary and secondary amines. Used in sequencing peptides by Edman degradation and in amino acid analyses by HPLC.

Confezionamento

One mL size packaged in flame sealed amber ampules under argon.
Ten mL size packaged in screw cap amber vials under argon.

Avvertenze

Danger

Indicazioni di pericolo

H301,H314,H317,H334

Classi di pericolo

Acute Tox. 3 Oral - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

190.4 °F - closed cup

Punto d’infiammabilità (°C)

88 °C - closed cup

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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R L Heinrikson et al.
Analytical biochemistry, 136(1), 65-74 (1984-01-01)
Methods for the quantitative derivatization of amino acids with phenylisothiocyanate and for the separation and quantitation of the resulting phenylthiocarbamyl derivatives by reverse-phase high-performance liquid chromatography are described. Phenylthiocarbamylation of amino acids proceeds smoothly in 5 to 10 min at
Examination of automated polypeptide sequencing using standard phenyl isothiocyanate reagent and subpicomole high-performance liquid chromatographic analysis
Tempst P and Riviere L
Analytical Biochemistry, 183(2), 290-300 (1989)
Additives for High Performance Applications: Chemistry and Applications
Additives for High Performance Applications: Chemistry and Applications, 235(1), 48-62 (2016)
The Protein Protocols Handbook, 235(1), 48-62 (1996)
H S Lu et al.
Archives of biochemistry and biophysics, 235(1), 48-62 (1984-11-15)
Automated solid-phase sequencing using 4-N,N-dimethylaminoazobenzene 4'-isothiocyanate (DABITC) double coupling or regular phenylisothiocyanate (PITC) degradation procedures have been investigated. Employing sensitive high-performance liquid chromatography for the identification of amino acid thiohydantoin derivatives (PTH and DABTH), both methods were capable of sequencing
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