E1257

Epidermal Growth Factor from murine submaxillary gland

lyophilized powder, BioReagent, suitable for cell culture

• Sinonimo/i: EGF, Epidermal cell growth factor
• Numero CAS: 62229-50-9
• Numero CE: 263-468-7
• Numero MDL: MFCD01634807
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: mouse submaxillary glands
• Livello qualitativo: 200
• Nome Commerciale: BioReagent
• Saggio: ≥98% (SDS-PAGE)
• Stato: lyophilized powder
• Potenza: 0.02-2.0 ng/mL EC₅₀
• Confezionamento: pkg of 0.1 mg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• tecniche: cell culture | mammalian: suitable
• Impurezze: endotoxin, tested
• Colore: white
• N° accesso UniProt: P01132
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: mouse ... Egf(13645)

Descrizione

Descrizione generale

Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, that binds to EGF receptor.[1] It is majorly expressed in kidney,[2] parotid gland and salivary gland.[3]

Applicazioni

Epidermal Growth Factor from murine submaxillary gland has been used

• in studying its effects on the development of caustic esophageal stricture in rats[4]
• for preparation of MCF-10A cell line[5]
• for the culture of primary hair follicle stem cells from arbas cashmere goat (gHFSCs) and adhesion of hair follicles (HF)[6]

Azioni biochim/fisiol

Epidermal growth factor (EGF) blocks gastric acid and pepsin secretion and increases gastrointestinal cell proliferation.[7] EGF stimulates proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes.[8] It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is linked to hypomagnesemia.[1] EGF plays a major role in the maintenance of hair and skin development.[9]

Stato fisico

Lyophilized from 0.2 μm filtered solution in ammonium acetate buffer.

Nota sulla preparazione

Purified by gel filtration and ion-exchange chromatography.

Risultati analitici

The biological activity is measured in a proliferation assay using BALB/MK cells.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)