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D8900

Sigma-Aldrich

Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sinonimo/i:

DME/F-12, miscela 1:1

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About This Item

Numero MDL:
MFCD00217417
Codice UNSPSC:
12352207
NACRES:
NA.71

Livello qualitativo

500

Forma fisica

powder

tecniche

cell culture | mammalian: suitable

Componenti

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Categorie correlate

Applicazioni

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:

  • as a component of the culture medium for endometrial explants in vitro culture
  • to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
  • to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein

Quantità

Formulated to contain 15.6 grams of powder per liter of medium.

Ricostituzione

Supplement with 1.2 g/L sodium bicarbonate.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

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Sang Hwan Kim et al.
PeerJ, 7, e6344-e6344 (2019-02-08)
Here we investigated the expressions of apoptosis-associated genes known to induce programed cell death through mRNA expressions of two matrix metalloproteinases (MMPs) that are involved in the degradation of collagen and basal membrane in luteal cells cultured in the treatment
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Cellular aggregates that mimic cell-cell interactions in vitro are essential for biological research. This study introduces a method to form large scaffold-free 3-D aggregates in a clinically ubiquitous cell culture dish using kilohertz-order ultrasound standing wave trapping (USWT). We fabricated
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Articoli

Nutrient Mixture F-12 Ham Formulation

Nutrient Mixture F-12 Ham Formulation

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Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.

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